| Product Name | Serum Amylase (AMY) Activity Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0030 |
| Description | Serum amylase (AMY) belongs to α -amylase and mainly exists in tissues and secretions such as the pancreas, salivary glands and small intestine. Its main function is to hydrolyze the α-1,4 glycosidic bonds within polysaccharide molecules in an irregular manner, generating a mixture of oligosaccharides, maltose and glucose for absorption and utilization by the body. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 48 Samples |
| Estimated Measurement Time | 4 h (48 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | Serum amylase catalyzes the hydrolysis of the α-1,4 glycosidic bond in the starch molecule to produce glucose, maltose, and dextrin, etc. Iodine can combine with unhydrolyzed starch to form a complex, and the product has a characteristic absorption peak at 570 nm, which can be characterized by the change of absorbance value. |
| Detection Methods | Starch-Iodine Colorimetric Method |
| Detection Wavelength | 570 nm |
| Signal Response | Decremental |
| Note | Absorbance measurement should be completed within 15 min after the color development is completed, if more samples are detected, it is recommended to perform the measurement in batches; if A measurement is outside the linear range of the standard absorbance value: higher than the highest value, it is recommended to dilute the crude enzyme solution appropriately and then perform the measurement; lower than the lowest value, it is recommended to appropriately prolong the enzyme reaction time or increase the sample volume and then perform the measurement, and the calculation is modified accordingly. |
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