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| Product Name | RT-LAMP Probe Master Mix in Lyophilized Beads |
| Catalog No. | NATR-HMM-0227 |
| Description | Alta DiagnoTech RT-LAMP probe master mix is a ready-to-reconstitute lyophilized beads for DNA or RNA isothermal amplification. |
| Intended Use | For DNA or RNA isothermal amplification. |
| Principle / Technology | A dried formulation supplies the core buffer and enzyme system; users add primer/probe mix and template before isothermal amplification. |
| Detection Method | Probe-based fluorescence detection |
| Reaction Conditions / Protocol | Use one dried dose with 5 µL 10× primer/probe mix and 45 µL RNA template. The 10× primer/probe mix contains 16 µM FIP/BIP, 2 µM F3/B3, 4 µM Loop F/B and 4 µM probe. Run 30 cycles at 65°C for 60 seconds with FAM fluorescence acquisition. The reaction temperature may be optimized from 62°C to 68°C. |
| Components / Formulation | Reaction buffer containing Mg2+ and dNTPs, Bst DNA polymerase and thermostable reverse transcriptase enzyme mix, lyophilization protectants. The 10× primer/probe mix contains 16 µM FIP/BIP, 2 µM F3/B3, 4 µM Loop F/B and 4 µM probe. |
| Storage Conditions | Store at 2-8°C. |
| Shelf Life | 12 months |
| Package Specifications | Available in 96-, 960- and 9,600-test formats, configured as 8 tests per strip with 12, 120 or 1,200 strips. |
| Product Form | Lyophilized beads |
| Shipping Conditions | Ship at 2-8°C. |
| Compatibility | Use nucleic-acid template dissolved in DEPC-treated water. Reaction temperature may be optimized for the selected primer set. |
| Application Notes / Precautions | Use a controlled workflow for reaction setup, sample handling and loading. Prepare the reaction mix in a clean bench and add template in a separate room or ventilated enclosure to reduce false-positive contamination. |
| Product Type | RT-LAMP probe master mix |
| Function / Efficacy | Supports probe-based isothermal amplification with FAM fluorescence acquisition. |
For research use only, not for clinical use.
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