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| Product Name | RNase H-Based Plant rRNA Depletion Kit (Cytoplasmic, Mitochondrial and Chloroplast rRNA) |
| Catalog No. | REKI-0054 |
| Description | An RNase H digestion kit that removes ribosomal RNA from plant total RNA, targeting cytoplasmic 18S and 25S rRNA, mitochondrial 18S and 26S rRNA and chloroplast 16S and 23S rRNA, to preserve messenger RNA and other non-coding RNA. It performs well on both intact and partially degraded total RNA and markedly raises the proportion of useful sequencing data. |
| Intended Use | rRNA removal before transcriptome sequencing of plant RNA, or before cDNA synthesis and other downstream applications. |
| Principle / Technology | Probe-guided RNase H specifically digests ribosomal RNA species while leaving mRNA and non-coding RNA intact. |
| Detection Method | Cytoplasmic 18S and 25S rRNA, mitochondrial 18S and 26S rRNA, chloroplast 16S and 23S rRNA |
| Sample Type | Plant total RNA, 1 ug input |
| Performance Range / Specifications | rRNA residual rate at or below 2%; high removal efficiency |
| Specificity | Broad plant species coverage with high sample compatibility |
| Storage Conditions | Store at -25 to -15°C |
| Package Specifications | 12 T / 24 T / 96 T |
| Product Form | Kit with RNase H and probe modules |
| Key Features | Enzymatic RNase H chemistry; chloroplast and mitochondrial targets included; compatible with any brand of total RNA library prep kit |
| Compatibility | Compatible with total RNA library prep kits from any supplier |
For research use only, not for clinical use.
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