Red Tracking RT SuperMix for qPCR with gDNA Removal
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Red Tracking RT SuperMix for qPCR with gDNA Removal

Cat.No: NATR-HMM-0174 Datasheet

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Product Name Red Tracking RT SuperMix for qPCR with gDNA Removal
Catalog No. NATR-HMM-0174
Description A two-stage qPCR reverse-transcription system that combines genomic DNA removal with a red tracking RT supermix for visible and reliable pipetting.
Intended Use First-strand cDNA synthesis; reverse transcription; gene expression analysis; two-step RT-qPCR.
Principle / Technology A genomic DNA removal mixture eliminates contaminating DNA before a red RT supermix converts RNA to cDNA.
Sample Type Total RNA.
Performance Range / Specifications 100 reactions; removes up to 500 ng of genomic DNA under the recommended conditions.
Specificity The red tracking dye supports visual pipetting without interfering with dye-based or probe-based qPCR.
Reaction Conditions / Protocol Treat RNA with the genomic DNA removal mixture, then add the red RT supermix and complete reverse transcription according to the protocol.
Components / Formulation 5× genomic DNA removal mix and 4× red RT supermix.
Storage Conditions Store at −30 to −15°C protected from light.
Package Specifications 100 reactions.
Product Form Two-stage red tracking RT supermix system.
Quality Control Qualified by genomic DNA removal, reverse transcription and subsequent qPCR analysis.
Key Features Visible red tracking; removal of up to 500 ng genomic DNA; simple two-stage workflow; dye- and probe-qPCR compatibility.
Shipping Conditions Ship at or below 0°C.
Compatibility Compatible with dye-based and probe-based qPCR assays.
Application Notes / Precautions Protect the red tracking supermix from light and mix gently before use.

For research use only, not for clinical use.

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