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| Product Name | Recombinase Polymerase Amplification (RPA) Lyophilized Reaction Pellet Kit |
| Catalog No. | NATR-HMM-0144 |
| Description | A lyophilized reagent kit for isothermal nucleic acid amplification using the recombinase polymerase amplification method. Each reaction pellet contains all essential enzymes and cofactors pre-dispensed in a dry, room-temperature-stable format requiring only the addition of primers, template, and magnesium acetate for initiation. RPA operates at a constant low temperature, eliminating the need for thermal cycling equipment. This format is suited for point-of-need and field-deployable molecular testing workflows. |
| Intended Use | Isothermal amplification of DNA targets at constant low temperature for rapid nucleic acid detection outside of laboratory settings. |
| Principle / Technology | Recombinase polymerase amplification at constant 37–42 °C |
| Detection Method | Real-time fluorescence, lateral flow dipstick, or gel electrophoresis |
| Sample Type | Purified DNA from clinical specimens, environmental samples, food matrices, or cultured organisms |
| Performance Range / Specifications | Amplification time: 10–20 min to detectable signal; compatible with 80–300 bp amplicons |
| Sensitivity / LOD | Detection limit: 1–10 copies of target DNA per reaction |
| Specificity | Primer-dependent target specificity; T4 UvsX recombinase and Bsu DNA polymerase function optimally at 39 °C |
| Reaction Conditions / Protocol | Rehydrate pellet with 29.5 µL rehydration buffer plus primers; add 2.5 µL magnesium acetate to initiate; incubate 20 min at 39 °C |
| Components / Formulation | Lyophilized RPA pellets (8-strip tube format), rehydration buffer, magnesium acetate solution (280 mM), positive control template and primers |
| Storage Conditions | Lyophilized pellets: room temperature for up to 3 weeks or 2–8 °C for long-term; rehydration buffer and magnesium acetate: –20 °C |
| Shelf Life | 12 months at 2–8 °C in sealed pouch with desiccant |
| Package Specifications | 96 reactions (12 × 8-strip tubes) |
| Product Form | Lyophilized pellet in 0.2 mL PCR tube strips |
| Quality Control | Each pellet lot validated with control template to confirm amplification within 15 min; NTC contamination check negative |
| Key Features | Room-temperature shipping and short-term storage removes cold chain dependency for field deployment |
| Purity | DNase/RNase free; PCR inhibitor free; A260/A280 ≥1.8 for nucleic acid products |
| Concentration | As specified on product label; enzyme units per µL as stated |
| Activity / Unit Definition | Polymerase activity defined as nmol dNTP incorporated per 30 min per unit; other activities per product specification |
| Molecular Weight | As specified per DNA markers or protein components |
| Source / Origin | Recombinant enzymes expressed in E. coli; synthetic oligonucleotides; ultrapure reagents |
| pH Range / Optimal pH | pH 7.5–8.5 for PCR; pH 8.0 for most molecular biology applications |
| Shipping Conditions | Cold packs or dry ice for enzymes; ambient for buffers and DNA markers |
| Expiration Date / Stability | 12–24 months at –20 °C for enzymes; 12–24 months at room temperature for buffers |
| Regulatory / Compliance | Research use; ISO 13485 manufacturing for select products |
| Compatibility | Compatible with standard thermal cyclers, real-time PCR instruments, and gel electrophoresis systems |
| Recommended Buffer System | Tris-HCl, KCl, MgCl₂-based reaction buffer; TE for nucleic acid storage |
| Application Notes / Precautions | Use dedicated PCR workspace; aliquot enzymes to avoid contamination; monitor no-template controls |
| Batch-to-Batch Consistency | Enzyme activity within ±15% of reference lot; PCR performance verified with control template |
For research use only, not for clinical use.
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