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| Product Name | Rapid Single- and Dual-Site Mutagenesis Kit V2 |
| Catalog No. | NATR-HMM-0166 |
| Description | A high-fidelity amplification and seamless recombination system for introducing mutations at one site or two separated sites in a target plasmid. |
| Intended Use | Single- and dual-site plasmid mutagenesis. |
| Principle / Technology | High-fidelity exponential plasmid amplification is followed by methylated-template digestion and homologous recombination-mediated recircularization. |
| Sample Type | Plasmid DNA up to 20 kb. |
| Performance Range / Specifications | Supports one mutation site or two noncontiguous sites separated by more than 50 bp; suitable for plasmids up to 20 kb. |
| Specificity | High-fidelity amplification reduces the probability of unwanted mutations during plasmid replication. |
| Reaction Conditions / Protocol | Amplify the target plasmid with mutagenic primers, digest the parental template with DpnI, then use the digestion product directly in the recombination reaction. |
| Components / Formulation | High-fidelity DNA amplification module and seamless recombination module. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 10 reactions; 25 reactions. |
| Product Form | Rapid site-directed mutagenesis kit. |
| Key Features | Low template input; direct post-digestion recombination; supports single and dual mutations; compatible with plasmids up to 20 kb; simplified primer design. |
| Compatibility | Dual-site mutations require the two target sites to be separated by more than 50 bp. |
For research use only, not for clinical use.
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