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| Product Name | Rapid Cell and Tissue Total RNA Isolation Kit |
| Catalog No. | DREK-0113 |
| Description | A rapid total RNA spin-column kit for animal cells and tissues. Optimized chemistry removes genomic DNA during the extraction workflow without requiring on-column DNase digestion. |
| Intended Use | Total RNA preparation from animal cells and tissues for RT-PCR, real-time PCR and microarray analysis. |
| Principle / Technology | Chemical lysis, integrated genomic DNA reduction and selective silica-membrane RNA purification. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Animal tissues and cultured animal cells. |
| Performance Range / Specifications | Approximately 6-minute room-temperature workflow; 50 reactions. |
| Reaction Conditions / Protocol | Lyse and homogenize the sample, process through the integrated DNA-removal workflow, bind RNA to the spin column, wash and elute. |
| Package Specifications | 50 reactions. |
| Product Form | Spin-column total RNA isolation kit. |
| Key Features | Six-minute workflow; reduced genomic DNA carryover without on-column DNase digestion; broad cell and tissue compatibility; no beta-mercaptoethanol, phenol or chloroform. |
| Compatibility | RT-PCR, real-time PCR and microarray workflows. |
| Application Notes / Precautions | Use RNase-free consumables and avoid overloading the column. Purified RNA should be aliquoted and stored frozen to minimize repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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