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| Product Name | Purple Universal Anti-Contamination SYBR qPCR Master Mix |
| Catalog No. | NATR-HMM-0188 |
| Description | A purple universal SYBR qPCR master mix using a dUTP and heat-labile UDG system to remove carryover products before amplification. |
| Intended Use | Relative and absolute nucleic acid quantification by dye-based real-time PCR. |
| Principle / Technology | Heat-labile UDG removes uracil-containing carryover DNA at room temperature and is inactivated during initial heating, while a hot-start polymerase drives specific qPCR amplification. |
| Detection Method | Intercalating-dye real-time PCR. |
| Sample Type | DNA or cDNA templates. |
| Performance Range / Specifications | 500 or 2,500 reactions; carryover removal above 99.99% was demonstrated under the evaluated conditions. |
| Specificity | Antibody-mediated hot-start blockade remains effective up to approximately 55°C, helping suppress nonspecific amplification. |
| Reaction Conditions / Protocol | Add primers and template to the master mix. The carryover-control enzyme acts at room temperature and is inactivated during heating at approximately 50–55°C before qPCR amplification. |
| Components / Formulation | qPCR master mix containing hot-start DNA polymerase, intercalating dye, dUTP, heat-labile UDG, universal passive-reference dye and purple tracking dye. |
| Storage Conditions | Store at −30 to −15°C protected from light. |
| Package Specifications | 500 reactions; 2,500 reactions. |
| Product Form | Purple universal anti-contamination 2× qPCR master mix. |
| Key Features | Room-temperature carryover control; heat-labile UDG; universal passive-reference system; high sensitivity and specificity; visible pipetting. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | After thawing, the master mix remains stable for up to 6 months at 2–8°C when protected from light. |
| Compatibility | Compatible with common real-time PCR instruments using or not using passive-reference normalization. |
For research use only, not for clinical use.
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