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| Product Name | Purple Tracking Anti-Contamination SYBR qPCR Master Mix |
| Catalog No. | NATR-HMM-0187 |
| Description | A purple tracking dye-based qPCR master mix combining hot-start amplification, universal passive-reference compatibility and dUTP/UDG carryover control. |
| Intended Use | Relative gene-expression analysis; absolute quantification; low-abundance target detection; amplification of complex or high-GC templates. |
| Principle / Technology | An antibody-mediated hot-start DNA polymerase, optimized qPCR buffer, intercalating dye and dUTP/UDG system support specific fluorescence-based amplification while reducing carryover contamination. |
| Detection Method | Intercalating-dye real-time PCR. |
| Sample Type | DNA or cDNA templates, including low-input, low-expression, partially degraded or high-GC samples. |
| Specificity | The hot-start polymerase provides strong 3′-end mismatch discrimination and remains effectively blocked at temperatures up to approximately 55°C. |
| Reaction Conditions / Protocol | Add primers and template to the supplied master mix and run a compatible real-time PCR program. |
| Components / Formulation | qPCR master mix containing hot-start DNA polymerase, intercalating dye, dUTP/UDG carryover-control system, universal passive-reference dye and purple tracking dye. |
| Storage Conditions | Store at −30 to −15°C protected from light. |
| Package Specifications | 500 reactions; 2,500 reactions. |
| Product Form | Purple tracking 2× qPCR master mix. |
| Key Features | Visible pipetting; carryover contamination control; universal passive-reference compatibility; high sensitivity; strong performance with challenging templates. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with common real-time PCR platforms using or not using passive-reference normalization. |
For research use only, not for clinical use.
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