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| Product Name | Protein A/G Magnetic Beads, Recombinant, 2 µm, Mixed Isotype Binding |
| Catalog No. | MAGBEA-0026 |
| Description | Superparamagnetic beads conjugated with recombinant Protein A/G fusion protein combining the IgG-binding domains of both Protein A and Protein G. This mixed-specificity reagent binds all major IgG subclasses from human, mouse, rabbit, goat, rat, sheep, and other species with high affinity. Ideal for laboratories using antibodies from multiple host species. |
| Intended Use | Universal immunoprecipitation across IgG subclasses and species; antibody purification from serum/ascites; Co-IP with diverse antibody sources; antibody screening and characterization. |
| Principle / Technology | Recombinant Protein A/G fusion protein (combining 5 IgG-binding domains from Protein A and 2 from Protein G) covalently coupled to superparamagnetic beads; recognizes Fc region of diverse IgG subclasses |
| Detection Method | 25-50 µL beads per IP; wash; incubate with antibody 10-30 min to pre-bind, then add lysate; or direct incubation with antibody-lysate mixture; magnetic separate; wash; elute |
| Components / Formulation | Protein A/G magnetic beads (10 mg/mL in PBS, 0.1% BSA, 0.02% NaN₃) |
| Storage Conditions | 2-8 °C; do not freeze; mix before use |
| Shelf Life | 24 months |
| Package Specifications | 1 mL, 5 mL, 10 mL suspension |
| Product Form | Brown-black magnetic suspension, 10 mg/mL |
| Key Features | Recombinant Protein A/G — no animal-derived antibodies; binds IgG from >10 species; high binding capacity >15 µg human IgG per mg beads; uniform 2 µm particles; low leaching; batch-to-batch consistency superior to animal-derived Protein A/G |
| Purity | Recombinant Protein A/G >98% SDS-PAGE; endotoxin <0.05 EU/mg; no non-specific protein contamination |
| Concentration | 10 mg beads/mL; >15 µg human IgG binding/mg beads |
| Activity / Unit Definition | Binding capacity in µg target per mg beads as specified |
| Molecular Weight | Not applicable — inorganic/organic composite particles |
| Source / Origin | Recombinant Protein A/G expressed in E. coli; animal component-free beads |
| pH Range / Optimal pH | pH 6.0–9.0 working range; optimal pH varies by surface chemistry |
| Shipping Conditions | Cold pack 2-8 °C; do not freeze |
| Expiration Date / Stability | 24 months at 2-8 °C; never freeze |
| Regulatory / Compliance | For laboratory and research use only; RUO; ACF; ISO 9001 |
| Compatibility | Binds human IgG1-4, mouse IgG1-2a-2b-3, rabbit IgG, goat IgG, rat IgG, sheep IgG, cow IgG, horse IgG, guinea pig IgG; weak binding to chicken IgY; no binding to IgM, IgA, IgE |
| Recommended Buffer System | PBS pH 7.4 with 0.02% sodium azide or 20% ethanol for long-term storage |
| Application Notes / Precautions | Vortex thoroughly before each use; avoid drying beads; use appropriate magnetic separation times |
| Batch-to-Batch Consistency | Particle size CV ≤10%; binding capacity within ±15% of reference lot |
For research use only, not for clinical use.
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