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| Product Name | Protein A Antibody Purification Kit |
| Catalog No. | SM-HMM-0056 |
| Description | The Protein A/G antibody purification magnetic beads series products utilize nano-surface technology to encapsulate Protein A/G onto the surface of superparamagnetic microspheres in a high-density orientation. Compared with similar products in the current international immunization magnetic bead market, this product has higher antibody binding capacity and lower protein non-specific adsorption rate, more uniform elution conditions, and the purity of the antibody can be separated from serum samples with a purity greater than 90% in one step purification. The product is nano-sized magnetic microspheres with large specific surface area, which can greatly shorten the time required for antibody adsorption, and skilled operation can complete the antibody adsorption process within 10min, and complete the antibody purification process within 30min. The antibody purification magnetic beads kit is equipped with optimized pre-made buffer, which provides the best reaction conditions for antibody purification experiments and improves the stability of antibody purification experiments. |
| Features | Highly efficient antibody binding capacity and ultra-low non-specific adsorption; Time-saving, easy and gentle operatio; High product stability; Antibody elution system closer to neutrality; Extremely low Protein A ligand shedding. Reusable. |
| Storage | 2-8°C |
| Shelf Life | 2 years |
| Average Particle Size | 2 µm |
| Concentration | 30 mg/mL |
Antibody purification is a critical step in life science research, as high-purity antibodies are essential for applications such as Western blotting, immunohistochemistry, enzyme-linked immunosorbent assays (ELISAs), and flow cytometry. Impure antibodies can lead to false results, reduced assay sensitivity, and compromised experimental reproducibility, making reliable purification tools indispensable for researchers worldwide.
Traditional antibody purification methods, such as column chromatography, often involve complex workflows, long processing times, and high non-specific adsorption, which limit efficiency and scalability. Magnetic bead-based purification has emerged as a game-changing alternative, offering faster processing, simpler operation, and better compatibility with high-throughput applications. Among magnetic bead-based solutions, Protein A/G-functionalized beads are widely favored due to their specific binding to the Fc region of immunoglobulins (IgG) from various species, enabling targeted and efficient antibody isolation.
The Protein A Antibody Purification Kit is developed to address the evolving needs of modern research. By integrating advanced nano-surface technology and optimized buffer systems, it overcomes the limitations of conventional methods, delivering high-purity antibodies in a streamlined workflow. Designed exclusively for research use (not for clinical applications), this kit provides researchers with a reliable, time-saving tool to support a wide range of life science studies, from basic research to drug discovery and development.
High-density orientation of Protein A on superparamagnetic microspheres, ensuring strong and specific binding to IgG antibodies.
Ultra-low non-specific adsorption rate, minimizing contamination from other proteins in serum or cell culture supernatants.
Rapid adsorption process that can be completed within 10 minutes, significantly reducing experimental time compared to traditional methods.
Total purification workflow finished in just 30 minutes, enabling high-throughput sample processing and improving research efficiency.
Antibody elution system close to neutral pH, preserving the structural integrity and biological activity of purified antibodies.
Extremely low Protein A ligand shedding, avoiding interference with downstream experimental applications.
Reusable magnetic beads, offering cost-effectiveness for long-term research projects.
Pre-optimized buffer solutions included, eliminating the need for time-consuming buffer preparation and ensuring consistent experimental results.
Stable performance with a 2-year shelf life when stored at 2-8°C, providing long-term reliability for laboratory use.
Nano-sized microspheres (2 µm average particle size) with a large specific surface area, enhancing antibody binding capacity and reaction kinetics.
Superior antibody binding capacity compared to standard immunomagnetic beads on the market, enabling efficient purification even from low-concentration antibody samples.
One-step purification achieves antibody purity greater than 90%, eliminating the need for additional polishing steps and simplifying experimental workflows.
Gentle and easy-to-operate protocol that does not require specialized equipment or technical expertise, suitable for both experienced researchers and laboratory beginners.
High product stability ensured by optimized formulation and storage conditions, reducing batch-to-batch variation and ensuring consistent performance.
Compatibility with a wide range of sample types, including serum, plasma, cell culture supernatants, and hybridoma supernatants, meeting diverse research needs.
Preservation of antibody activity through mild elution conditions, ensuring purified antibodies are suitable for subsequent functional assays and applications.
Cost-effective design with reusable beads and pre-made buffers, lowering overall experimental costs without compromising quality.
Streamlined workflow that integrates seamlessly into high-throughput research pipelines, supporting large-scale experimental projects and accelerating research progress.
For research use only, not for clinical use.
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