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| Product Name | Polysaccharide- and Polyphenol-Rich Plant Total RNA Isolation Kit |
| Catalog No. | DREK-0114 |
| Description | A silica-column kit optimized for total RNA isolation from difficult plant tissues rich in polysaccharides and polyphenols. Separate workflows and a dedicated genomic DNA/impurity filter improve purification across diverse plant matrices. |
| Intended Use | Plant total RNA isolation for RT-PCR, real-time PCR, microarray analysis, Northern blotting, dot blotting, poly(A) selection, in vitro translation, RNase protection assays and molecular cloning. |
| Principle / Technology | Plant-specific chemical lysis, impurity and genomic DNA filtration, selective silica-membrane RNA binding, washing and elution. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Plant tissues rich in polysaccharides or polyphenols, including leaf, needle, fruit, flower and tuber samples. |
| Performance Range / Specifications | Approximately 30-minute workflow; 50 reactions. |
| Reaction Conditions / Protocol | Select the workflow appropriate for the plant matrix, grind tissue thoroughly, lyse, remove impurities and genomic DNA through the filter column, bind RNA to the RNA column, wash and elute. |
| Components / Formulation | Genomic DNA and impurity filter column, silica RNA purification column and optimized plant lysis/wash reagents. |
| Storage Conditions | Store at 15-25°C. |
| Package Specifications | 50 reactions. |
| Product Form | Plant total RNA spin-column kit. |
| Key Features | Optimized for polysaccharide- and polyphenol-rich plants; two sample-processing workflows; effective impurity and genomic DNA removal; high-purity RNA. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | RT-PCR, real-time PCR, blotting, microarray, poly(A) selection, in vitro translation and cloning workflows. |
| Application Notes / Precautions | Use the sample-specific workflow and ensure complete grinding in liquid nitrogen. Avoid excessive sample input and prolonged exposure before lysis. |
For research use only, not for clinical use.
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