- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Polybrene Transduction Enhancement Reagent, 10 mg/mL |
| Catalog No. | ATR-CTR-0021 |
| Description | Sterile 10 mg/mL polybrene (hexadimethrine bromide) solution for lentiviral and retroviral transduction enhancement. The cationic polymer neutralizes charge repulsion between virions and cell surface, increasing transduction efficiency 2-10 fold. Essential for stable cell line generation and CRISPR sgRNA library delivery via lentiviral vectors. |
| Intended Use | Lentiviral/retroviral transduction enhancement; stable cell line generation; CRISPR sgRNA library transduction; improvement of low-titer viral preparations. |
| Principle / Technology | Polybrene polycation neutralizes electrostatic repulsion between negatively charged virion envelope and cell surface heparan sulfate proteoglycans, facilitating virus adsorption and membrane fusion |
| Detection Method | Add to culture medium at 4-8 µg/mL final before viral transduction; incubate 4-24 hr; wash and replace medium |
| Components / Formulation | Hexadimethrine bromide, 10 mg/mL in sterile 0.9% NaCl; 0.22 µm filtered |
| Storage Conditions | 2-8 °C; protect from light; do not freeze; sterile — handle aseptically |
| Shelf Life | 24 months; 12 months after opening |
| Package Specifications | 0.5 mL, 1 mL, 5 mL sterile vials |
| Product Form | Clear colorless sterile liquid |
| Key Features | Sterile and ready-to-use; validated for lenti/retrovirus; 2-10× efficiency increase; endotoxin <0.1 EU/mg; consistent lot-to-lot concentration |
| Purity | Polybrene ≥95%; endotoxin <0.1 EU/mg; sterility tested USP <71> |
| Concentration | 10 mg/mL in sterile 0.9% NaCl |
| Activity / Unit Definition | Transfection efficiency: ≥70% in HEK293 cells with control plasmid at recommended ratio |
| Molecular Weight | Average Mn ~5,000-10,000 Da |
| Source / Origin | Synthetic cationic lipids and polymers; recombinant viral components where applicable |
| pH Range / Optimal pH | Lipoplex formation optimal at pH 7.0–7.4 |
| Shipping Conditions | Ambient; protect from light; do not freeze |
| Expiration Date / Stability | 24 months at 2-8 °C; 12 months after opening |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with serum-containing and serum-free culture media; check product-specific compatibility |
| Recommended Buffer System | Serum-free medium or PBS for complex formation; complete medium for post-transfection culture |
| Application Notes / Precautions | Optimize reagent-to-DNA ratio per cell type; avoid antibiotic during transfection; monitor cytotoxicity |
| Batch-to-Batch Consistency | Transfection efficiency within ±15% of reference lot on HEK293 standard |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
