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| Product Name | Polybrene (Hexadimethrine Bromide, 10 mg/mL) |
| Catalog No. | RESK-0273 |
| Description | Polybrene (hexadimethrine bromide) is a polycationic polymer commonly used in DNA transfection of mammalian cells to enhance liposome-mediated transfection efficiency. Polybrene is currently widely used in retrovirus-mediated and lentivirus-mediated gene transduction; its mechanism of action is thought to involve neutralizing the electrostatic repulsion between sialic acid on the cell surface and viral particles, thereby promoting viral adsorption. Polybrene is also a well-known anti-heparin agent (heparin antagonist) and is often used to produce non-specifically agglutinated red blood cells. In addition, Polybrene is frequently used in protein sequencing, because small amounts of Polybrene markedly reduce peptide degradation in automated sequencing analysis. Adding Polybrene to PVDF membranes can also improve membrane affinity. This product is supplied as a solution: the powder is dissolved in 0.9% NaCl to prepare a 10 mg/mL solution, which is sterilized by filtration through a 0.22 um filter. For use, it is generally diluted 1:1000-1:2000; the dilution ratio varies depending on the cell type, and the relevant literature should be consulted. |
| Intended Use | Enhancement of retrovirus- and lentivirus-mediated gene transduction; enhancement of liposome-mediated DNA transfection in mammalian cells; anti-heparin (heparin antagonist) applications; protein sequencing support; improvement of PVDF membrane affinity. For research use only. |
| Principle / Technology | As a cationic polymer, Polybrene interacts with the negative charges on the surface of viral vectors (e.g., lentivirus), neutralizing the electrostatic repulsion between cell-surface sialic acid and viral particles, thereby increasing viral particle adhesion to cells and significantly improving infection efficiency. |
| Sample Type | Mammalian cell cultures for viral transduction or DNA transfection. |
| Performance Range / Specifications | Enhances transfection efficiency: as a cationic polymer, Polybrene interacts with negative charges on the surface of viral vectors (e.g., lentivirus), increasing adhesion of viral particles to cells and significantly improving viral infection efficiency. Good stability: stable performance when stored at 2-8 °C, with a long shelf life, ensuring reliable results during experiments. Easy to use: no complex pretreatment is required; it can be added directly at a defined ratio into the culture medium containing the viral vector, with simpler handling than other transfection reagents or methods. |
| Reaction Conditions / Protocol | Generally used at a 1:1000-1:2000 dilution of the 10 mg/mL stock; the exact ratio depends on the cell type and should be determined according to the relevant literature. Example: for a 10 mL experimental system at a 1:1000 ratio, add 10 uL of the 10 mg/mL product directly into the system. |
| Components / Formulation | Polybrene (hexadimethrine bromide) dissolved in 0.9% NaCl at 10 mg/mL; sterilized by filtration through a 0.22 um filter. |
| Storage Conditions | Store at -20 °C for up to 2 years. Aliquoting is recommended to avoid repeated freeze-thaw cycles. Stable performance when stored at 2-8 °C. |
| Shelf Life | 2 years when stored at -20 °C. |
| Package Specifications | 500 uL 5x 500 uL |
| Product Form | Sterile-filtered liquid solution (10 mg/mL in 0.9% NaCl). |
| Quality Control | Filter-sterilized through a 0.22 um filter; stable performance verified during storage. |
| Concentration | 10 mg/mL |
| Source / Origin | Hexadimethrine bromide, a synthetic polycationic polymer. |
| Shipping Conditions | Shipped with wet ice. |
| Expiration Date / Stability | Stable for 2 years at -20 °C; aliquot to avoid repeated freeze-thaw cycles. |
| Compatibility | Compatible with retroviral and lentiviral vectors and with liposome-mediated DNA transfection protocols; working dilution is cell-type dependent. |
| Application Notes / Precautions | Dilution refers to the ratio at which the product is added to the experimental system, not dilution with another solvent. Typical working dilution is 1:1000-1:2000 (e.g., 10 uL of the 10 mg/mL stock per 10 mL of system at 1:1000); the optimal ratio varies with cell type and should be confirmed in the relevant literature. Aliquot upon receipt to avoid repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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