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| Product Name | Poly(A)-Tailing miRNA First-Strand cDNA Synthesis Kit |
| Catalog No. | NATR-HMM-0180 |
| Description | A one-tube miRNA reverse-transcription system that combines poly(A) tailing and cDNA synthesis for convenient downstream miRNA analysis. |
| Intended Use | First-strand cDNA synthesis for miRNA detection and quantification. |
| Principle / Technology | Poly(A) polymerase adds poly(A) tails to miRNA or total RNA, followed by reverse transcription using a universal primer and an optimized miRNA reverse-transcription system. |
| Sample Type | Purified miRNA or total RNA. |
| Specificity | The optimized system is designed to reduce reverse transcription of precursor miRNA while supporting mature miRNA analysis. |
| Reaction Conditions / Protocol | Perform poly(A) tailing and reverse transcription in a single tube according to the recommended reaction program. |
| Components / Formulation | Poly(A) tailing reagents, miRNA reverse-transcription reagents, universal reverse-transcription primer and RNase inhibitor. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 20 reactions; 50 reactions. |
| Product Form | miRNA first-strand cDNA synthesis kit. |
| Key Features | One-tube tailing and reverse transcription; convenient universal-primer workflow; optimized mature-miRNA detection; RNase protection included. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Resulting cDNA is suitable for downstream miRNA qPCR analysis. |
For research use only, not for clinical use.
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