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| Product Name | Plasmid DNA Mini Extraction Kit |
| Catalog No. | DREK-0099 |
| Description | A routine plasmid miniprep kit using optimized alkaline lysis and selective spin-column purification. The process does not require phenol/chloroform extraction, alcohol precipitation or column equilibration. |
| Intended Use | Plasmid DNA purification for restriction digestion, PCR, sequencing, ligation and transformation. |
| Principle / Technology | SDS-alkaline lysis followed by selective silica-membrane binding, washing and elution. |
| Detection Method | Downstream plasmid DNA analysis. |
| Sample Type | 1-5 mL overnight bacterial culture. |
| Performance Range / Specifications | Approximately 30-minute workflow; column binding capacity up to 35 µg plasmid DNA; compatible with plasmids demonstrated from approximately 3-10 kb; 100 reactions. |
| Specificity | Selective plasmid DNA recovery with removal of protein, genomic DNA, RNA and other bacterial impurities. |
| Reaction Conditions / Protocol | Harvest the culture, resuspend, lyse and neutralize, clarify the lysate, bind plasmid DNA to the spin column, wash and elute. |
| Components / Formulation | Resuspension, lysis, neutralization, wash and elution reagents with RNase, silica spin columns and collection tubes. |
| Storage Conditions | Store at 15-25°C. After RNase is added to the resuspension buffer, store at 2-8°C for up to 6 months. |
| Package Specifications | 100 reactions. |
| Product Form | Plasmid DNA spin-column kit. |
| Quality Control | Yield, DNA integrity, purity and downstream restriction-digestion performance are evaluated during release testing. |
| Key Features | No column equilibration; approximately 30-minute workflow; up to 35 µg binding capacity; high-purity plasmid DNA. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | Restriction digestion, PCR, sequencing, ligation and transformation workflows. |
| Application Notes / Precautions | For low-copy or >10 kb plasmids, increase culture input and optimize elution as directed; mix alkaline lysates gently to reduce genomic DNA carryover. |
| Batch-to-Batch Consistency | Plasmid yield and purity are controlled across production batches. |
For research use only, not for clinical use.
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