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| Product Name | PiggyBac Transposon Transfection System for Stable Integration |
| Catalog No. | ATR-CTR-0012 |
| Description | A non-viral stable gene transfer system consisting of a transposon donor plasmid and a transposase expression helper plasmid for the efficient, stable genomic integration of large transgene cassettes in mammalian cells. |
| Intended Use | Generation of stable cell lines through transposon-mediated genomic integration of large transgenes (up to 100 kb); suitable for multi-gene expression systems, inducible expression constructs, and cell line engineering without viral vector biosafety concerns. |
| Principle / Technology | PiggyBac transposase recognizes terminal inverted repeat sequences flanking the transgene cargo on the donor plasmid, excises the cargo, and catalyzes its integration at TTAA tetranucleotide sites in the host genome with precise footprint-free excision capability. |
| Detection Method | Stable integration verified by antibiotic selection and colony counting, transgene copy number by qPCR, integration site analysis by splinkerette PCR |
| Sample Type | Mammalian cell lines: HEK293, HeLa, CHO, iPSCs, ESCs, primary T cells |
| Performance Range / Specifications | Integration efficiency 5–20% for plasmid co-transfection (colonies per seeded cells); cargo capacity >100 kb; stable expression maintained >50 passages |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Co-transfect donor plasmid and transposase helper plasmid at 2:1 to 5:1 ratio using standard transfection method; apply antibiotic selection 48 hours post-transfection; expand resistant colonies. |
| Components / Formulation | PiggyBac donor plasmid (with multiple cloning site), PiggyBac transposase expression plasmid (CMV-PBase), bacterial stabs |
| Storage Conditions | -20°C (plasmid DNA) |
| Shelf Life | 36 months at -20°C |
| Package Specifications | 10 µg donor plasmid, 10 µg transposase plasmid, bacterial stabs |
| Product Form | Plasmid DNA in TE buffer |
| Quality Control | Plasmid sequence verification, transposase activity by excision assay, integration efficiency validation in HEK293 |
| Key Features | Large cargo capacity; genomic integration; stable long-term expression; non-viral; validated for multi-gene constructs; precise excision capability |
For research use only, not for clinical use.
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