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| Product Name | Phosphofructokinase (PFK) Activity Assay Kit, 100T/96S |
| Catalog No. | SCAK-YJL-0206 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, utilizing ATP. This irreversible step is the primary rate-limiting and regulatory point of glycolysis. PFK activity is allosterically controlled by numerous metabolites (e.g., ATP, citrate, AMP, fructose-2,6-bisphosphate), making it a central hub for cellular energy sensing and metabolic flux control. This kit measures PFK activity by coupling the ADP produced in the reaction to the oxidation of NADH, which is monitored by the decrease in absorbance at 340 nm. |
| Product Application | • Research on glycolysis regulation, metabolic flux, and bioenergetics. • Studies of metabolic disorders, cancer metabolism (Warburg effect), and insulin resistance. • Characterization of allosteric regulation and inhibitor screening for PFK. • Investigation of tissue-specific glycolytic capacity (e.g., muscle, liver, tumor). |
| Product Advantages | • Coupled Kinetic Assay: Provides a continuous, real-time measurement of PFK activity, allowing for accurate determination of initial velocities and kinetic parameters. • High Sensitivity: The coupled enzyme system amplifies the signal, enabling detection of PFK activity in samples with low enzyme abundance or limited material. • Flexible for Allosteric Studies: The assay can be easily adapted by adding various effectors (ATP, AMP, citrate, etc.) to study the complex allosteric regulation of PFK. • Comprehensive Kit: Includes all necessary substrates, coupling enzymes (pyruvate kinase, lactate dehydrogenase), and cofactors optimized for reliable PFK activity measurement. |
For research use only, not for clinical use.
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