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| Product Name | Lacate Dehydrogenase (LDH) Activity Assay Kit, 100T/48S, 200T/96S |
| Catalog No. | SCAK-YJL-0037 |
| Detection Method | Micro-volume method |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Lactate dehydrogenase (LDH) is a stable cytoplasmic enzyme present in nearly all cells. It catalyzes the interconversion of pyruvate and lactate with concomitant oxidation/reduction of NADH/NAD⁺. The release of LDH into the extracellular space is a well-established marker of cellular membrane damage and cytotoxicity, while its intracellular activity is key to anaerobic glycolysis. This kit measures LDH activity by monitoring the oxidation of NADH to NAD⁺, which is coupled to a colorimetric probe, allowing flexible application for both activity assays in lysates and release assays in culture supernatants. |
| Product Application | • Quantitative assessment of cell death, cytotoxicity, and drug toxicity in vitro. • Evaluation of tissue injury in models of ischemia, infarction, or trauma (e.g., cardiac, liver). • Research on cellular metabolism, particularly glycolysis, the Warburg effect in cancer, and hypoxia. • Monitoring of fermentation processes in industrial microbiology. |
| Product Advantages | • Dual-Use Design: Suitable for both intracellular LDH activity measurement and supernatant-based cytotoxicity/viability testing. • Scalable Formats: Available in 100T/48S and 200T/96S configurations to match different lab throughput needs. • Sensitive & Linear: Provides a wide dynamic range for accurate detection of subtle changes in enzyme levels. • Robust Performance: Consistent results across diverse sample types including cell lysates, serum, and tissue homogenates. |
For research use only, not for clinical use.
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