- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Phenol-Guanidinium Total RNA Isolation Reagent |
| Catalog No. | DREK-0103 |
| Description | A single-phase phenol/guanidinium reagent for strong sample lysis and rapid RNase inactivation. Chloroform partitioning followed by isopropanol precipitation yields high-quality total RNA from diverse biological samples. |
| Intended Use | Total RNA isolation for RT-PCR, qRT-PCR, Northern blotting, dot blotting, in vitro translation and high-throughput sequencing. |
| Principle / Technology | Phenol/guanidinium lysis, chloroform-induced phase separation, recovery of the aqueous RNA phase, isopropanol precipitation and ethanol washing. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Cultured cells, animal tissues, plant tissues, large fungi and compatible biological samples. |
| Performance Range / Specifications | Strong lysis and RNase-inactivation capacity with high RNA yield; supplied in 100 mL and 200 mL formats. |
| Specificity | Phase partitioning separates RNA into the aqueous phase while DNA and protein remain in the interphase and organic phase. |
| Reaction Conditions / Protocol | Homogenize the sample in the reagent, add chloroform, centrifuge at 2-8°C, transfer the aqueous phase, precipitate RNA with isopropanol, wash with 75% ethanol and dissolve. |
| Components / Formulation | Ready-to-use single-phase phenol/guanidinium RNA isolation reagent. |
| Package Specifications | 100 mL; 200 mL. |
| Product Form | Liquid phenol/guanidinium RNA isolation reagent. |
| Quality Control | RNA yield, purity and integrity are evaluated across representative cell, animal tissue and plant samples. |
| Key Features | Strong lysis; broad sample compatibility; high RNA yield; rapid RNase inactivation. |
| Compatibility | RT-PCR, qRT-PCR, blotting, in vitro translation and sequencing workflows. |
| Application Notes / Precautions | Use appropriate chemical protection and work in a ventilated area. Perform phase separation at 2-8°C and avoid disturbing the interphase when collecting the aqueous layer. |
| Batch-to-Batch Consistency | RNA yield and integrity are controlled across production batches. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
