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| Product Name | Peptide-Based Cellular Delivery Reagent, Endoporter System |
| Catalog No. | ATR-CTR-0011 |
| Description | A membrane-permeable peptide-based delivery system designed to transport proteins, peptides, and other macromolecules into the cytoplasm of living cells through a non-endocytic, direct membrane translocation mechanism. |
| Intended Use | Intracellular delivery of functional proteins, antibodies, and peptide-based probes into live cells for protein complementation assays, intracellular epitope tagging, and functional studies requiring native protein delivery. |
| Principle / Technology | Amphipathic peptide with leucine-rich hydrophobic face forms transient membrane perturbations that facilitate direct translocation of cargo across the plasma membrane into the cytoplasm without endosomal entrapment; cargo is rapidly released into the reducing environment of the cytoplasm. |
| Detection Method | Protein delivery confirmed by immunofluorescence against delivered protein or functional assay of delivered enzyme activity |
| Sample Type | Living mammalian cells of all types: cell lines, primary cells, stem cells, organoids |
| Performance Range / Specifications | Protein delivery efficiency >50% of cells at 5–10 µM cargo concentration; functional enzyme activity within 1 hour of delivery |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Mix protein or peptide cargo with Endoporter reagent in PBS; incubate 5–15 min at room temperature; add to cells in serum-free medium for 30–60 min; wash cells; add complete medium; assay activity within 1–24 hours. |
| Components / Formulation | Amphipathic membrane transduction peptide, 1 mM in DMSO, sterile-filtered |
| Storage Conditions | -20°C, protect from moisture and light; thaw and aliquot for single use |
| Shelf Life | 12 months at -20°C; use within 1 month after thawing |
| Package Specifications | 0.5 mg, 2 mg, 10 mg |
| Product Form | Lyophilized or DMSO solution |
| Quality Control | Peptide purity by HPLC >95%, protein delivery efficiency validation with GFP or beta-galactosidase cargo, cytotoxicity testing |
| Key Features | Non-endocytic delivery; functional protein delivery; rapid cytoplasmic access; compatible with diverse cargo sizes; validated for antibodies, enzymes, and transcription factors |
For research use only, not for clinical use.
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