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| Product Name | One-Step Seamless Cloning Kit |
| Catalog No. | NATR-HMM-0162 |
| Description | A one-tube seamless cloning kit for rapid directional assembly of single or multiple DNA fragments into linearized vectors. |
| Intended Use | Rapid cloning, high-throughput cloning, seamless cloning, site-directed mutagenesis and double-stranded DNA assembly. |
| Principle / Technology | A ligase-independent homologous recombination system assembles inserts and vectors carrying 15–20 bp matching terminal sequences. |
| Sample Type | Linearized vectors and one to five DNA inserts. |
| Performance Range / Specifications | Supports one to five fragments and individual inserts from 50 bp to 10 kb. |
| Specificity | Reported positive clone rate exceeds 95%. |
| Reaction Conditions / Protocol | Generate 15–20 bp homologous ends, then incubate the recombination reaction at 50°C for 5–15 minutes before transformation. |
| Components / Formulation | 2× seamless cloning mix; linearized zero-background dual-resistance vector (50 ng/μL); 500 bp control insert (20 ng/μL). |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 25 reactions; 50 reactions. |
| Product Form | One-step seamless cloning kit. |
| Key Features | Five- to fifteen-minute recombination; one-tube operation; one- to five-fragment compatibility; 50 bp to 10 kb insert range; low vector self-ligation background. |
| Compatibility | Compatible with single- and multi-fragment assembly and unpurified high-fidelity PCR products. |
| Application Notes / Precautions | The supplied linearized vector carries ampicillin and kanamycin resistance and may be sequenced with a universal M13 primer. |
For research use only, not for clinical use.
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