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| Product Name | Nuclease-Free Water (Molecular Biology Grade) |
| Catalog No. | NATR-HMM-0124 |
| Description | Ultra-pure, sterile water that has been processed to remove detectable DNase, RNase, and protease activities. The water undergoes multiple purification steps including deionization, reverse osmosis, UV treatment, and terminal filtration under strict quality control, producing water suitable for the most sensitive nucleic acid applications. |
| Intended Use | Preparation of PCR, qPCR, RT-PCR, and RT-qPCR reaction mixtures; elution of DNA and RNA from purification columns; reconstitution of lyophilized nucleic acids, primers, and probes; dilution of nucleic acid samples; preparation of all molecular biology reagents and buffers requiring nuclease-free conditions. |
| Principle / Technology | Multi-stage purification including activated carbon filtration, ion exchange, reverse osmosis, ultraviolet photo-oxidation (185 and 254 nm), and 0.1 μm terminal membrane filtration. Each lot is tested for the absence of nucleases that would degrade DNA or RNA during incubation. |
| Detection Method | Conductivity measurement for ionic purity; DNase/RNase activity testing with fluorescent or radioactive nucleic acid substrates; endotoxin testing by LAL assay; microbial count testing |
| Sample Type | Reagent for all molecular biology applications; compatible with enzymatic reactions including PCR, restriction digestion, ligation, reverse transcription, in vitro transcription, and sequencing |
| Performance Range / Specifications | Resistivity: ≥18.2 MΩ·cm at 25°C; conductivity: ≤0.055 μS/cm; TOC (Total Organic Carbon): ≤10 ppb; endotoxin: ≤0.005 EU/mL; particulates: ≤1 particle/mL (>0.22 μm); pH: 6.8–7.2 |
| Sensitivity / LOD | No detectable DNase activity: incubation of 1 μg lambda DNA with test water for 16 hours at 37°C shows no degradation; no detectable RNase activity: incubation of 1 μg MS2 RNA for 4 hours at 37°C shows no degradation |
| Specificity | Free of DNase, RNase, and non-specific endonucleases and exonucleases; no PCR inhibitors detected in functional amplification assays; absence of bacterial, fungal, and phage contamination |
| Reaction Conditions / Protocol | Ready to use; no preparation required; aseptically dispensed under laminar flow; use directly for any molecular biology procedure; aliquot into smaller volumes to maintain sterility for long-term use |
| Components / Formulation | Ultra-pure deionized water, processed by multi-stage purification and sterile filtered (0.1 μm); supplied in molecular biology grade HDPE or PETG bottles certified DNase/RNase-free |
| Storage Conditions | Room temperature (15–25°C); protect from direct sunlight and excessive heat; once opened, store tightly capped; for applications requiring the highest nuclease-free assurance, aliquot and store at –20°C |
| Shelf Life | 36 months from date of manufacture in unopened container; after opening, use within 6 months for most sensitive applications |
| Package Specifications | 500 mL, 1 L, and 4 L bottles; also available in 50 mL aliquots for single-use convenience |
| Product Form | Sterile-filtered liquid |
| Quality Control | Each lot tested: resistivity ≥18.2 MΩ·cm; DNase-free validated by 16-hour DNA incubation; RNase-free validated by 4-hour RNA incubation; endotoxin ≤0.005 EU/mL; microbial count <1 CFU/mL; pH 6.5–7.5; no PCR inhibition in qPCR assay with 50% water content |
| Key Features | Triple-tested for DNase, RNase, and protease absence; ultrapure specifications exceed USP Water for Injection standards; endotoxin-tested for cell culture and in vivo work; multiple bottle sizes for laboratory workflow flexibility; validated for next-generation sequencing sample preparation |
For research use only, not for clinical use.
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