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| Product Name | NADP/NADPH Assay Reagent |
| Catalog No. | CMTR-HMM-0018 |
| Description | NADP (nicotinamide adenine dinucleotide phosphate) is a coenzyme formed by the coupling of ribosyl nicotinamide 5-phosphate (NMN) with 5-phosphoadenosine 2,5-diphosphate via a pyrophosphate bond. It acts as an electron carrier in many reactions, alternately being oxidized (NADP+) and reduced (NADPH). |
| Application | This product is used to detect intracellular nucleotides (NADP, NADPH, and their ratio). |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 2 h |
| Sample Type | Cell lysate, Tissue extracts |
| Procedure | 1. Extract samples from cells/tissues using extraction buffer and perform protein removal using a spin column. 2. For NADPH measurement, heat the sample to 60°C and maintain for 30 minutes to decompose NAD+, then cool on ice (this step is not required for total NADP+/NADPH measurement). 3. Add the sample and standard to the wells. 4. Add the reaction mixture and incubate at room temperature for 5 minutes to convert NADP to NADPH. 5. Add the NADPH chromogen and incubate for 1–4 hours while performing reaction cycles. 6. Analyze multiple times during the 1–4 hour incubation period using an enzyme-linked immunosorbent assay (ELISA) reader. 7. The reaction can be stopped using a stop solution. |
| Storage | -20°C |
For research use only, not for clinical use.
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