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| Product Name | Multi-Fragment One-Step Seamless Cloning Kit |
| Catalog No. | NATR-HMM-0164 |
| Description | A ligase-independent seamless cloning kit optimized for ordered assembly of two to five DNA fragments into a linearized vector. |
| Intended Use | Rapid cloning, high-throughput cloning, seamless cloning, site-directed mutagenesis and multi-fragment DNA assembly. |
| Principle / Technology | Enhanced recombinase and optimized buffer drive homologous assembly of multiple inserts while reducing vector self-ligation. |
| Sample Type | Linearized vectors and two to five DNA fragments. |
| Performance Range / Specifications | Supports ordered assembly of two to five insert fragments in a single reaction. |
| Specificity | Reported positive clone rate reaches 95% or higher. |
| Reaction Conditions / Protocol | Incubate the recombination reaction for 30 minutes before transformation. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 10 reactions; 25 reactions. |
| Product Form | Multi-fragment seamless cloning kit. |
| Key Features | Two- to five-fragment assembly; no dependence on internal restriction sites; accepts unpurified linearized vectors and PCR products; low self-ligation background. |
| Compatibility | Compatible with ordered multi-fragment assembly into a wide range of linearized vectors. |
For research use only, not for clinical use.
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