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| Product Name | mRNA Transfection Reagent |
| Catalog No. | RESK-0276 |
| Description | This cationic polymer-based transfection reagent is designed for cellular mRNA transfection and delivers high transfection efficiency in conventional cell lines, primary cells, and neural cells. Its unique formulation allows it to be added directly to the culture medium, and the presence of serum does not affect transfection efficiency, avoiding cell damage caused by serum removal. After transfection, there is no need to remove the nucleic acid-reagent complexes or replace the medium; fresh medium may optionally be added 4-6 hours later depending on the nutritional status of the cells. The reagent is a colorless, clear, water-soluble solution; the nanoparticles it forms with RNA remain stable at room temperature for at least 30 minutes, ensuring experimental reproducibility and result consistency. It performs excellently in mRNA, siRNA, and gene-editing applications, achieving gene editing efficiencies of up to 95% in vitro, and is easy to use under standard laboratory conditions without complex instrumentation. |
| Intended Use | For research use in the transfection of mRNA into mammalian cells, including conventional cell lines, primary cells, and neural cells; also applicable to siRNA delivery and gene-editing experiments. |
| Principle / Technology | A cationic polymer forms nanoscale complexes with negatively charged RNA molecules. The nanoparticles protect the RNA and mediate its cellular uptake, enabling efficient intracellular delivery and expression. |
| Sample Type | mRNA; also suitable for siRNA and gene-editing payloads |
| Performance Range / Specifications | High transfection efficiency in conventional, primary, and neural cells Up to 95% gene editing efficiency achievable in vitro RNA-reagent nanoparticles stable at room temperature for at least 30 minutes Tolerates physiological buffers and serum; effective across pH 4-8 Colorless, clear, water-soluble formulation Serum-compatible; no medium change required after transfection |
| Specificity | Optimized for mRNA transfection; also validated for siRNA and gene-editing applications |
| Reaction Conditions / Protocol | Add the transfection complex directly to cells in serum-containing medium. If transfection performance is suboptimal, place the prepared transfection complex on a shaker (900 rpm, 25 °C) for 30 seconds, then let stand for about 5 minutes; the complex remains stable within 30 minutes. No medium change is required after transfection; fresh medium may be added 4-6 hours after transfection depending on cell nutritional status. |
| Components / Formulation | Cationic polymer-based transfection reagent; colorless, clear, water-soluble liquid |
| Storage Conditions | Store at 2-8 °C. Do not freeze. |
| Shelf Life | 1 year when stored at 2-8 °C |
| Package Specifications | 0.1 mL 1 mL |
| Product Form | Colorless, clear liquid |
| pH Range / Optimal pH | Functional across pH 4-8; tolerant of physiological buffers and serum |
| Shipping Conditions | Shipped on wet ice (ice packs) |
| Expiration Date / Stability | Valid for 1 year when stored at 2-8 °C. Do not freeze. Prepared RNA-reagent complexes are stable at room temperature for at least 30 minutes. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic procedures. |
| Compatibility | Compatible with serum-containing medium, physiological buffers, and a wide range of cell types including hard-to-transfect primary and neural cells |
| Application Notes / Precautions | Do not freeze. Use prepared transfection complexes within 30 minutes for best results. No medium change is required after transfection, reducing handling stress on cells. |
For research use only, not for clinical use.
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