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| Product Name | Micro-Input Cell and Tissue Total RNA Isolation Kit |
| Catalog No. | DREK-0107 |
| Description | A micro-input silica-column kit for rapid total RNA isolation from very small animal cell and tissue samples. Dedicated DNA-removal and micro-RNA-binding columns reduce genomic DNA carryover and support low-volume elution. |
| Intended Use | Micro-input total RNA preparation for RT-PCR, qRT-PCR, Northern blotting, dot blotting, in vitro translation and high-throughput sequencing. |
| Principle / Technology | Chemical lysis, dedicated genomic DNA removal and selective silica-membrane RNA binding followed by washing and low-volume elution. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Animal cells and tissues, with input as low as approximately 10 cells or 1 µg tissue. |
| Performance Range / Specifications | Approximately 5-minute extraction; input as low as 10 cells or 1 µg tissue; elution volume as low as 10 µL; 50 reactions. |
| Sensitivity / LOD | Designed for RNA recovery from micro-input samples down to approximately 10 cells. |
| Specificity | A dedicated DNA-removal column reduces genomic DNA carryover before RNA purification. |
| Reaction Conditions / Protocol | Lyse the micro-input sample, remove genomic DNA using the dedicated filter, bind RNA to the micro column, wash and elute in a low volume. |
| Components / Formulation | Lysis and binding reagents, genomic DNA removal filters, micro-RNA spin columns, wash and elution reagents, and carrier RNA. |
| Storage Conditions | Store the temperature-sensitive component set at -30 to -15°C and the room-temperature component set at 15-25°C. |
| Package Specifications | 50 reactions. |
| Product Form | Micro-input total RNA spin-column kit. |
| Quality Control | Low-input recovery, RNA integrity and downstream RT-qPCR performance are evaluated with representative cell and tissue samples. |
| Key Features | Input down to 10 cells; approximately 5-minute workflow; elution down to 10 µL; reduced genomic DNA carryover; phenol-free and β-mercaptoethanol-free handling. |
| Shipping Conditions | Ship the frozen component set at 0°C or below and the room-temperature set at ambient temperature. |
| Compatibility | RT-PCR, qRT-PCR, blotting, in vitro translation and sequencing workflows. |
| Application Notes / Precautions | Use strict RNase-free technique and minimize sample loss during transfer. Very small inputs require careful contamination control. |
| Batch-to-Batch Consistency | Micro-input RNA recovery and RT-qPCR performance are controlled across production batches. |
For research use only, not for clinical use.
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