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| Product Name | Maltase/α-Gucosidase (α-GC) Activity Assay Kit, 100T/48S |
| Catalog No. | SCAK-YJL-0223 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
α-Glucosidase (α-GC), also commonly referred to as maltase in specific contexts, is a key hydrolase enzyme that plays a critical role in carbohydrate metabolism. It catalyzes the hydrolysis of α-1,4 glycosidic bonds in disaccharides (such as maltose) and oligosaccharides, releasing glucose as the end product. This enzymatic reaction is essential for understanding fundamental biological processes, including energy metabolism, cellular nutrient utilization, and the regulation of carbohydrate breakdown in various organisms.
In research settings, the accurate detection and quantification of α-GC activity are vital across multiple fields. These include biochemistry, molecular biology, pharmacology, and food science. For example, in pharmaceutical research, α-GC activity assays are widely used to screen potential inhibitors for the development of drugs targeting type 2 diabetes, as inhibiting this enzyme can slow down carbohydrate digestion and reduce postprandial blood glucose levels. In food science, the assay helps evaluate the efficiency of carbohydrate hydrolysis in food processing or the quality of fermented products.
Traditional methods for measuring α-GC activity often suffer from limitations such as low sensitivity, complex operation steps, long reaction times, or interference from sample matrices. The demand for reliable, efficient, and high-throughput assay tools has driven the development of specialized kits that address these challenges, enabling researchers to obtain accurate and reproducible results with minimal experimental effort.
Utilizes a micro-volume detection method that requires only small sample volumes, conserving valuable research materials while ensuring high sensitivity.
Compatible with both spectrophotometers and microplate readers, offering flexibility to adapt to different laboratory equipment setups and throughput requirements.
Features a stable storage condition of -20°C with a 6-month shelf life, ensuring long-term usability and reducing the need for frequent reordering.
Designed with a user-friendly workflow that minimizes complex preparation steps, allowing researchers to complete assays efficiently within a reasonable time frame.
Provides specific and selective detection of α-GC activity, reducing interference from other enzymes or components in biological samples for more reliable data.
High accuracy and reproducibility: The assay kit is optimized to deliver consistent results across multiple experiments and sample batches, enhancing the reliability of research data.
Time and labor saving: The simplified experimental procedure eliminates the need for tedious reagent preparation and lengthy incubation steps, streamlining the workflow for researchers.
Broad sample applicability: Suitable for analyzing α-GC activity in various sample types, including tissue homogenates, cell lysates, enzyme preparations, and biological fluids, meeting the diverse needs of different research projects.
Cost-effective: The kit provides sufficient reagents for 100 or 48 assays (depending on the format), offering a cost-efficient solution for both small-scale experiments and large-scale screening studies.
Safety-focused design: Reminds users to wear appropriate protective equipment during operation, ensuring compliance with laboratory safety standards and protecting researchers’ health.
For research use only, not for clinical use.
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