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| Product Name | Magrose NHS Kit |
| Catalog No. | SM-HMM-0034 |
| Description | Magrose NHS Kit is a porous microsphere based on natural hydrophilic polymer agarose with a particle size of 10-30 µm, paired with the buffer required for coupling. The NHS group on the surface of the beads is capable of forming stable peptide bonds with proteins and other molecules with a primary amine group for affinity purification or analytical detection of antibodies, antigens and other biomolecules. |
| Features | Simple, no need for activation, can be directly covalently coupled with biological ligands; High efficiency, the coupling efficiency of biological ligands can reach more than 90%, which is much higher than that of carboxylated magnetic beads, and the binding load of ligands is higher at the same time; Rapid, 1~2 h to complete the coupling of biological ligands; Mild, room temperature or 4°Ccoupling, the pH of the coupling system is 5~9; Stable, forming stable amide bonds, preventing ligand detachment. Good biocompatibility, reduce non-specific adsorption. |
| Average Particle Size | 10-30 µm |
| Preservation Fluid | Anhydrous isopropyl alcohol |
| Concentration | 20% (v/v) |
| Applications | In Vitro Diagnostics
Immunoassays Cell Sorting Immunoprecipitation/co-precipitation Protein/antibody Isolation and Purification |
The development of affinity purification and analytical detection technologies has become increasingly critical in life sciences, biotechnology, and in vitro diagnostic research. Researchers and industry professionals require reliable tools to efficiently isolate, purify, and analyze biomolecules such as antibodies, antigens, and proteins—key components in experiments ranging from basic research to diagnostic assay development.
Magnetic microspheres have emerged as a cornerstone in these applications due to their ease of manipulation, high surface area, and compatibility with various coupling chemistries. Among them, agarose-based magnetic microspheres stand out for their natural hydrophilicity and excellent biocompatibility, which minimize non-specific adsorption and preserve the activity of sensitive biomolecules.
The Magrose NHS Kit is designed to address the growing demand for streamlined, high-performance coupling solutions. By integrating NHS (N-hydroxysuccinimide) functional groups onto porous agarose microspheres, the kit eliminates the need for complex pre-activation steps, allowing for direct covalent coupling with primary amine-containing molecules. This design aligns with the industry’s shift toward time-efficient, user-friendly tools that do not compromise on coupling efficiency or stability.
Tailored for research use exclusively (not for clinical applications), the kit supports a wide range of research scenarios, including immunoassays, cell sorting, immunoprecipitation, and protein/antibody purification, providing a versatile solution for laboratories and research institutions worldwide.
Ready-to-use functionality: No prior activation is required, enabling direct covalent coupling with biological ligands containing primary amine groups.
Exceptional coupling efficiency: Achieves over 90% coupling efficiency for biological ligands, outperforming many conventional carboxylated magnetic beads.
High ligand binding capacity: Offers a higher load of bound ligands compared to alternative magnetic bead systems, supporting more efficient purification and detection.
Rapid processing: Completes the entire ligand coupling process in just 1–2 hours, significantly reducing experimental turnaround time.
Mild reaction conditions: Operates at room temperature or 4°C with a coupling system pH range of 5–9, protecting the structural and functional integrity of biomolecules.
Stable bond formation: Forms covalent amide bonds between ligands and microspheres, preventing ligand detachment during washing or experimental procedures.
Superior biocompatibility: Constructed from natural hydrophilic agarose, minimizing non-specific adsorption and ensuring optimal performance in biomolecular interactions.
Well-defined particle size: Features an average particle size of 10–30 µm, balancing efficient magnetic responsiveness and uniform interaction with target molecules.
Convenient preservation: Supplied in anhydrous isopropyl alcohol to maintain product stability during storage and transportation.
Simplified workflow: Eliminates tedious activation steps, reducing experimental complexity and the risk of human error.
Cost-effective performance: High coupling efficiency and ligand load translate to lower material usage per experiment, optimizing research budgets.
Versatile application scope: Compatible with multiple research techniques, including in vitro diagnostics research, immunoassays, cell sorting, and protein purification.
Biomolecule protection: Mild reaction conditions preserve the activity of sensitive proteins, antibodies, and antigens, ensuring reliable and reproducible results.
Long-term reliability: Stable amide bonds and high-quality agarose matrix ensure consistent performance across multiple experiments and storage cycles.
User-friendly design: Suitable for both experienced researchers and laboratory personnel new to magnetic bead-based coupling, with straightforward operation requirements.
Research-focused optimization: Exclusively engineered for research use, with specifications tailored to meet the rigorous demands of academic and industrial research.
For research use only, not for clinical use.
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