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| Product Name | Magnetic Bead-Based microRNA Extraction Kit |
| Catalog No. | DREK-0027 |
| Description | Specialized magnetic bead-based kit for enrichment and purification of microRNA (miRNA) and other small RNA species (<200 nt) from total RNA, cells, tissues, and biofluids. Proprietary binding chemistry preferentially captures small RNA molecules while depleting large RNA species (>200 nt), mRNA, and rRNA. Extracted miRNA is suitable for miRNA-seq library preparation, RT-qPCR miRNA profiling, and microarray analysis. |
| Intended Use | miRNA and small RNA extraction from total RNA samples; miRNA enrichment from cells, tissues, plasma/serum, and exosomes; circulating miRNA biomarker discovery; small RNA sequencing library preparation. |
| Principle / Technology | Selective binding chemistry: small RNAs (<200 nt) preferentially bind to magnetic beads under optimized buffer conditions; large RNAs remain in solution and are removed; bound small RNAs washed and eluted. Starting from total RNA, miRNA enrichment factor >50-fold |
| Detection Method | Mix total RNA or cell/tissue lysate with binding buffer and magnetic beads; incubate 10 min; magnetic separate (large RNAs in supernatant discarded); wash 2× with small RNA wash buffer; elute miRNA in 20-50 µL nuclease-free water |
| Sample Type | Total RNA (1-10 µg), cultured cells (10⁵-10⁷), fresh/frozen tissue (10-50 mg), plasma/serum (200-500 µL), exosomes, biofluids |
| Sensitivity / LOD | Recovers miRNA from as little as 100 ng total RNA input; yields 20-200 ng enriched small RNA from 1 µg total RNA; effective for low-abundance miRNAs at <10 copies per cell |
| Specificity | Small RNA selective (<200 nt): enriches miRNA (~22 nt), piRNA (~26-31 nt), tiRNA, and snoRNA; depletes >90% of mRNA and rRNA |
| Reaction Conditions / Protocol | Binding: 10 min RT; washes: 2× 5 min; elution: 5 min; total protocol ~30 min post-lysis |
| Components / Formulation | Small RNA binding buffer, small RNA magnetic beads (25 mg/mL), small RNA wash buffer 1, small RNA wash buffer 2, nuclease-free elution water, collection tubes (1.5 mL, nuclease-free) |
| Storage Conditions | 15-25 °C; magnetic beads do not freeze; keep bottles tightly closed |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | 20 preps, 50 preps, 100 preps |
| Product Form | Liquid reagents, ready-to-use |
| Key Features | Preferential small RNA capture (<200 nt); >50-fold miRNA enrichment from total RNA; magnetic bead format for automation; includes nuclease-free water; protocol ~30 min; extracted miRNA compatible with miRNA-seq, RT-qPCR (stem-loop or polyA-tailing), and microarray |
| Purity | No detectable large RNA carryover (Bioanalyzer confirmed); no RNase activity detected; endotoxin <0.01 EU/µL eluate |
| Concentration | Elution volume and yield as specified per sample type |
| Activity / Unit Definition | DNA/RNA binding capacity per column or per mg beads as specified |
| Molecular Weight | Genomic DNA >50 kb; RNA 0.1–10 kb range as applicable |
| Source / Origin | Silica membrane or magnetic bead technology; recombinant Proteinase K |
| pH Range / Optimal pH | Binding buffer pH 5.0–7.0 for silica-based binding |
| Shipping Conditions | Ambient temperature |
| Expiration Date / Stability | 12 months at 15-25 °C; do not freeze beads; if beads aggregate, vortex >30 sec; discard if binding buffer develops turbidity |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with total RNA extracted by TRIzol, column, or magnetic bead methods; for biofluids, pre-concentrate using ethanol precipitation or spin concentrator; enriched miRNA compatible with all commercial miRNA-seq library prep kits |
| Recommended Buffer System | Chaotropic salt binding buffer; ethanol-based wash; low-salt TE or water elution |
| Application Notes / Precautions | Pre-warm elution buffer to improve yield; avoid cross-contamination between samples; change pipette tips between steps |
| Batch-to-Batch Consistency | DNA yield and purity within ±15% of reference lot on standardized blood sample |
For research use only, not for clinical use.
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