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| Product Name | Magnetic Bead-Based Fecal DNA Extraction Kit |
| Catalog No. | DREK-0026 |
| Description | Magnetic bead-based kit optimized for extraction of microbial genomic DNA from fecal, gut content, and intestinal biopsy samples. Includes a stool stabilization buffer for sample collection and transport. Advanced inhibitor removal handles complex polysaccharides, bile salts, and heme compounds characteristic of fecal samples. Extracted DNA is suitable for gut microbiome studies, 16S rRNA metagenomics, and pathogen detection. |
| Intended Use | Fecal/stool microbial DNA extraction; gut microbiome profiling; enteric pathogen detection; metagenomic shotgun sequencing; intestinal biopsy microbiome analysis; animal gut microbiota studies. |
| Principle / Technology | Stool stabilization buffer preserves microbial composition; mechanical bead beating + thermal/enzymatic lysis (lysozyme + proteinase K); inhibitor removal matrix adsorbs polyphenols, bile salts, and polysaccharides; magnetic beads bind DNA in optimized buffer; ethanol washes; elution in low-salt buffer |
| Detection Method | Homogenize 200 mg stool in stabilization buffer; transfer 500 µL to bead tube; bead beat 5 min; heat 70 °C 10 min with lysozyme; add proteinase K, 56 °C 10 min; centrifuge; add inhibitor removal matrix, incubate, centrifuge; add binding buffer and beads; wash 2×; elute 50-100 µL |
| Sample Type | Fresh, frozen, or stabilized fecal samples (200 mg); gut contents; intestinal biopsies; stool collected in stabilization buffer stable 7 days at RT, 30 days at 4 °C |
| Sensitivity / LOD | Yields 3-20 µg DNA per 200 mg stool; effective on both high-biomass (omnivore) and low-biomass (antibiotic-treated) samples; A260/A280 1.8-2.0 |
| Components / Formulation | Stool stabilization buffer, bead beating tubes (0.1 mm + 0.5 mm zirconia + 2 mm glass beads), lysozyme (50 mg/mL, lyophilized), proteinase K (20 mg/mL), inhibitor removal matrix, binding buffer, silica magnetic beads (50 mg/mL), wash buffer 1, wash buffer 2, elution buffer (Tris 10 mM, pH 8.0) |
| Storage Conditions | Lysozyme and proteinase K at -20 °C; remaining components at 15-25 °C |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | 50 preps, 100 preps |
| Product Form | Liquid reagents with bead beating tubes and lyophilized lysozyme |
| Key Features | Stool stabilization buffer for field collection; comprehensive inhibitor removal; bead beating + enzymatic lysis for Gram-positive bacteria; magnetic bead format for automation; DNA suitable for 16S, metagenomics, and qPCR without dilution; protocol ~90 min for 24 samples |
| Purity | A260/A280 1.8-2.0; A260/A230 >1.8; no detectable PCR inhibition at 1:5 dilution with universal 16S primers |
| Concentration | Elution volume and yield as specified per sample type |
| Activity / Unit Definition | DNA/RNA binding capacity per column or per mg beads as specified |
| Molecular Weight | Genomic DNA >50 kb; RNA 0.1–10 kb range as applicable |
| Source / Origin | Silica membrane or magnetic bead technology; recombinant Proteinase K |
| pH Range / Optimal pH | Binding buffer pH 5.0–7.0 for silica-based binding |
| Shipping Conditions | Ambient for most components; lysozyme and proteinase K -20 °C cold pack; stabilization buffer ambient |
| Expiration Date / Stability | 12 months; reconstituted lysozyme 2 weeks at 4 °C; proteinase K 12 months at -20 °C |
| Regulatory / Compliance | For laboratory and research use only; RUO; fecal samples are biohazardous — handle per BSL-2 guidelines; ISO 9001 |
| Compatibility | Compatible with human, mouse, rat, and livestock fecal samples; infant (meconium) and low-biomass samples process normally; extracted DNA compatible with Illumina, MGI, and PacBio sequencing platforms |
| Recommended Buffer System | Chaotropic salt binding buffer; ethanol-based wash; low-salt TE or water elution |
| Application Notes / Precautions | Pre-warm elution buffer to improve yield; avoid cross-contamination between samples; change pipette tips between steps |
| Batch-to-Batch Consistency | DNA yield and purity within ±15% of reference lot on standardized blood sample |
For research use only, not for clinical use.
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