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| Product Name | Magnetic Bead-Based Circulating RNA Extraction Kit |
| Catalog No. | DREK-0028 |
| Description | Magnetic bead-based kit for extraction of circulating cell-free RNA (cfRNA) including mRNA, miRNA, and other small non-coding RNAs from human plasma, serum, and other biofluids. Optimized for low-input, fragmented nucleic acids with carrier RNA-assisted binding. Extracted cfRNA is suitable for liquid biopsy applications including circulating transcriptome analysis, miRNA biomarker quantification, and non-invasive prenatal testing (NIPT). |
| Intended Use | Circulating cfRNA extraction from plasma/serum; liquid biopsy transcriptome and miRNA analysis; cancer biomarker discovery; non-invasive prenatal testing; cardiovascular disease biomarker studies; exosome RNA extraction. |
| Principle / Technology | Proteinase K digestion releases protein-bound RNA; optimized binding buffer with carrier RNA enhances recovery of low-concentration cfRNA; magnetic silica beads capture RNA; ethanol washes remove contaminants; elution in small volume maximizes concentration for downstream analysis |
| Detection Method | Digest 0.5-4 mL plasma with proteinase K (60 °C, 20 min); add binding buffer with carrier RNA and magnetic beads; incubate 10 min with mixing; magnetic separate; wash 2×; dry beads briefly; elute in 20-50 µL |
| Sample Type | Human plasma (EDTA preferred, citrate acceptable), serum, urine, CSF, bronchoalveolar lavage, ascites fluid; 0.5-4 mL input volume |
| Sensitivity / LOD | Recovers circulating RNA from 0.5 mL plasma; detects miRNA at <100 copies/mL plasma; linear recovery across 0.5-4 mL input range; carrier RNA enhances recovery 3-5 fold for low inputs |
| Specificity | Total RNA capture (mRNA, miRNA, lncRNA, piRNA); DNA carryover <1% (DNase I treatment optional); poly(A) carrier RNA does not interfere with downstream miRNA detection |
| Reaction Conditions / Protocol | Proteinase K digestion: 20 min 60 °C; binding: 10 min; washes: 2× 5 min; elution: 5 min; total ~50 min for up to 24 samples |
| Components / Formulation | Proteinase K (20 mg/mL), cfRNA binding buffer concentrate with carrier poly(A) RNA, silica magnetic beads (50 mg/mL), wash buffer 1, wash buffer 2, nuclease-free elution water, LoBind collection tubes (1.5 mL) |
| Storage Conditions | Proteinase K at -20 °C; remaining components at 15-25 °C |
| Shelf Life | 12 months; proteinase K 12 months at -20 °C |
| Package Specifications | 20 preps, 50 preps, 100 preps |
| Product Form | Liquid reagents; LoBind tubes for eluate collection |
| Key Features | Optimized for circulating cfRNA; carrier RNA maximizes recovery; magnetic bead format for automation; LoBind tubes prevent adsorption losses; proteinase K digestion releases protein-bound RNA; protocol <1 hour; eluate suitable for RT-qPCR, miRNA-seq, and transcriptome profiling |
| Purity | Carrier RNA synthetic, certified nuclease-free; eluate A260/A280 not applicable (carrier RNA contribution); no detectable PCR inhibition |
| Concentration | Elution volume and yield as specified per sample type |
| Activity / Unit Definition | DNA/RNA binding capacity per column or per mg beads as specified |
| Molecular Weight | Genomic DNA >50 kb; RNA 0.1–10 kb range as applicable |
| Source / Origin | Silica membrane or magnetic bead technology; recombinant Proteinase K |
| pH Range / Optimal pH | Binding buffer pH 5.0–7.0 for silica-based binding |
| Shipping Conditions | Cold pack for proteinase K; ambient for remaining components |
| Expiration Date / Stability | 12 months; proteinase K 12 months at -20 °C; binding buffer with carrier RNA stable 12 months at RT when unopened |
| Regulatory / Compliance | For laboratory and research use only; RUO; human plasma/serum biohazardous — handle per BSL-2; ISO 9001 |
| Compatibility | Compatible with manual spin columns, vacuum manifolds, and automated liquid handlers |
| Recommended Buffer System | Chaotropic salt binding buffer; ethanol-based wash; low-salt TE or water elution |
| Application Notes / Precautions | Pre-warm elution buffer to improve yield; avoid cross-contamination between samples; change pipette tips between steps |
| Batch-to-Batch Consistency | DNA yield and purity within ±15% of reference lot on standardized blood sample |
For research use only, not for clinical use.
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