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| Product Name | Low-Background Stable Multiplex Probe qPCR Premix |
| Catalog No. | NATR-HMM-0208 |
| Description | A concentrated 5× multiplex probe qPCR premix designed for low reagent-derived DNA background and long-term stability after complete primer/probe premixing. |
| Intended Use | Multiplex bacterial pathogen testing and detection of E. coli-related biological-product contaminants. |
| Principle / Technology | A high-specificity hot-start polymerase, dual-antibody blocking and optimized buffer suppress primer/probe interactions during storage, while background-DNA control and a dUTP/UDG system reduce false-positive contamination. |
| Detection Method | Multiplex fluorescent probe-based real-time PCR. |
| Sample Type | DNA templates from bacterial pathogen assays and compatible biological-product testing samples. |
| Performance Range / Specifications | 200 or 500 reactions; 5× premix; linear range of approximately 10–10⁵ copies/µL with amplification efficiency around 100% in the reported evaluation. |
| Sensitivity / LOD | Supports low-copy multiplex DNA detection across a broad quantitative range. |
| Specificity | Strong 3′ mismatch recognition and hot-start blocking limit nonspecific amplification and primer/probe cross-interactions. |
| Reaction Conditions / Protocol | Premix the 5× reagent with assay primers and probes, then add the DNA sample and run a compatible multiplex probe-qPCR program. |
| Components / Formulation | 5× multiplex probe qPCR premix containing hot-start Taq polymerase, optimized buffer, dUTP and UDG carryover-control chemistry. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 500 reactions. |
| Product Form | 5× low-background multiplex probe qPCR premix. |
| Quality Control | Reagent-background DNA, amplification efficiency, quantitative linearity, premix stability and carryover-control performance are evaluated. |
| Key Features | Low reagent-derived DNA background; stable full primer/probe premixing; approximately 100% amplification efficiency in the tested range; dUTP/UDG carryover control; simple sample-addition workflow. |
| Concentration | 5× premix. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | After primer/probe premixing, reported performance remained stable for 28 days at 37°C and 50 freeze–thaw cycles. |
| Compatibility | Compatible with common multichannel real-time PCR instruments used for multiplex probe assays. |
| Application Notes / Precautions | Use assay-specific primers and probes and include no-template controls to monitor background DNA and carryover contamination. |
For research use only, not for clinical use.
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