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| Product Name | Liquid Chromatography Column Cleaning Solution, Organic Residue Remover |
| Catalog No. | ATRCS-0026 |
| Description | Multi-step column cleaning solution set for HPLC and UPLC analytical columns to remove strongly retained hydrophobic contaminants, protein precipitates, and metal ion deposits. The three-solution system sequentially addresses organic residues (Solution A: acetonitrile-water with TFA), proteinaceous build-up (Solution B: guanidine-urea with reducing agent), and metal chelation (Solution C: EDTA-based stripping buffer). Regular use extends column lifetime and restores peak symmetry and resolution degraded by accumulated sample matrix components. |
| Intended Use | Routine maintenance and regeneration of reversed-phase, ion-exchange, and size-exclusion chromatography columns used in biomolecule analysis and purification. |
| Principle / Technology | Sequential chemical cleaning targeting hydrophobic interactions (organic solvent disruption), hydrogen bonding and protein aggregation (chaotropic disruption), and metal-chelate interactions (EDTA stripping) to remove adsorbed contaminants from column stationary phase. |
| Detection Method | Sequential flushing of column with Solutions A, B, and C at low flow rate (0.1-0.3 mL/min for analytical columns); UV monitoring at 214 nm and 280 nm to track contaminant elution. |
| Sample Type | Analytical and preparative HPLC/UPLC columns; applicable to C18, C8, C4, phenyl, ion-exchange, and size-exclusion phases. |
| Performance Range / Specifications | Restores up to 90% of original column efficiency (plate count) and 95% of original peak asymmetry factor in most cases. |
| Sensitivity / LOD | Able to remove contaminants causing baseline drift >0.5 mAU and peak tailing factor >2.0. |
| Specificity | Solution A targets hydrophobic residues; Solution B targets protein aggregates and H-bond networks; Solution C targets metal ion adducts. |
| Reaction Conditions / Protocol | Flush column with each solution for 10-20 column volumes at specified flow rate; monitor UV signal until baseline stabilizes before switching solutions. |
| Components / Formulation | Solution A: Acetonitrile/Water (50:50) with 0.1% TFA; Solution B: 6 M Guanidine HCl, 2 M Urea, 5 mM DTT; Solution C: 100 mM EDTA, pH 8.0. |
| Storage Conditions | Store at room temperature (15-25 °C); Solutions B and C protect from air oxidation after opening. |
| Shelf Life | Solution A: 24 months; Solution B: 12 months; Solution C: 24 months from date of manufacture. |
| Package Specifications | 100 mL each solution in amber glass bottles with PTFE-lined caps. |
| Product Form | Clear liquid solutions; Solution B is colorless to pale yellow. |
| Quality Control | Each solution lot tested for UV transparency (A214 and A280), pH verification, and absence of particulate matter. |
| Key Features | Three-solution sequential cleaning system; compatible with silica and polymer-based stationary phases; metal-free packaging; detailed cleaning protocol card included. |
| Purity | HPLC gradient grade solvents and reagents; EDTA and guanidine hydrochloride ≥99% purity; TFA spectrophotometric grade. |
| Concentration | Ready-to-use solutions; no further dilution required. |
| Activity / Unit Definition | Cleaning efficacy verified by restoration of column performance using protein test mixture (ribonuclease A, cytochrome c, BSA, carbonic anhydrase). |
| Molecular Weight | Not applicable — multi-component cleaning system. |
| Source / Origin | Synthetic organic solvents and reagents from analytical grade raw materials. |
| pH Range / Optimal pH | Solution A: pH ~2.0; Solution B: pH ~6.5; Solution C: pH 8.0 ± 0.1. |
| Shipping Conditions | Ambient temperature; Solution B ship with cold pack if transit exceeds 5 days in summer. |
| Expiration Date / Stability | As specified per solution; after opening, Solution B should be used within 3 months due to DTT oxidation. |
| Regulatory / Compliance | For laboratory use only; not for diagnostic or therapeutic applications. |
| Compatibility | Compatible with silica-based, polymer-based, and hybrid stationary phases. Not recommended for aminopropyl or diol bonded phases with extended exposure. |
| Recommended Buffer System | Solution C uses Tris-EDTA buffer; Solutions A and B are unbuffered organic/aqueous mixtures. |
| Application Notes / Precautions | Always reverse column flow direction during cleaning. Start with lowest organic solvent concentration and increase gradually. Monitor back-pressure throughout cleaning; discontinue if pressure exceeds column specifications. Re-equilibrate column with mobile phase after cleaning. Do not exceed column manufacturer temperature or pressure limits. |
| Batch-to-Batch Consistency | pH verification within ±0.2 units per solution; UV absorbance at 254 nm within ±0.01 AU of reference lot. |
For research use only, not for clinical use.
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