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| Product Name | Lipofectamine-Equivalent Cationic Lipid Transfection Reagent for Plasmid DNA |
| Catalog No. | ATR-CTR-0020 |
| Description | A multi-component cationic lipid formulation designed as a direct replacement for first-generation lipid transfection reagents used for plasmid DNA delivery into adherent mammalian cells. The reagent forms stable complexes with plasmid DNA through electrostatic interaction between the cationic lipid head groups and negatively charged DNA phosphate backbone. The lipid formulation includes a neutral co-lipid that stabilizes the lipoplex structure and enhances membrane fusion activity. |
| Intended Use | Transient transfection of adherent mammalian cells with plasmid DNA for recombinant protein expression and reporter gene assays. |
| Principle / Technology | Cationic lipid-mediated DNA condensation and lipoplex formation with endocytosis-mediated cell entry |
| Detection Method | Fluorescence microscopy, luciferase assay, or Western blot depending on plasmid construct |
| Sample Type | HEK293, HeLa, COS-7, NIH/3T3, CHO-K1, and other commonly used adherent cell lines |
| Performance Range / Specifications | Transfection efficiency: 70–90% in HEK293 with pEGFP-N1; suitable DNA range: 0.5–5 µg per 10⁶ cells |
| Sensitivity / LOD | GFP expression detectable by flow cytometry at 12 hr post-transfection in HEK293 |
| Specificity | Broad activity across standard adherent cell lines; less effective in suspension and primary cells |
| Reaction Conditions / Protocol | Mix DNA and reagent in serum-free medium at 1:2 to 1:4 (µg DNA:µL reagent) ratio; incubate 15 min at room temperature; add complexes to cells in complete medium; assay 24–48 hr post-transfection |
| Components / Formulation | Cationic lipid reagent in 70% ethanol, serum-free dilution medium, enhancer solution for some protocols |
| Storage Conditions | 2–8 °C for lipid reagent; do not freeze; protect from moisture |
| Shelf Life | 12 months at 2–8 °C in sealed vial |
| Package Specifications | 1 mL, 5 mL (sufficient for 300–1500 transfections in 24-well format) |
| Product Form | Liquid lipid mixture in ethanol |
| Quality Control | Transfection efficiency and cytotoxicity verified on HEK293, HeLa, and NIH/3T3 reference cell lines per lot |
| Key Features | Direct replacement for legacy first-generation reagents, maintaining established protocols with comparable or improved performance |
| Purity | Endotoxin-free; sterile-filtered; certified nuclease-free |
| Concentration | As specified (typically 1 mg/mL for lipid reagents) |
| Activity / Unit Definition | Transfection efficiency: ≥70% in HEK293 cells with control plasmid at recommended ratio |
| Molecular Weight | Varies by formulation as specified in product documentation |
| Source / Origin | Synthetic cationic lipids and polymers; recombinant viral components where applicable |
| pH Range / Optimal pH | Lipoplex formation optimal at pH 7.0–7.4 |
| Shipping Conditions | Cold packs; some formulations require –20 °C; do not freeze lipid emulsions |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use only; biosafety level 1 for standard transfection; BSL-2 for viral systems |
| Compatibility | Compatible with serum-containing and serum-free culture media; check product-specific compatibility |
| Recommended Buffer System | Serum-free medium or PBS for complex formation; complete medium for post-transfection culture |
| Application Notes / Precautions | Optimize reagent-to-DNA ratio per cell type; avoid antibiotic during transfection; monitor cytotoxicity |
| Batch-to-Batch Consistency | Transfection efficiency within ±15% of reference lot on HEK293 standard |
For research use only, not for clinical use.
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