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| Product Name | Linear Polyethylenimine (PEI), MW 40,000, Rapid-Dissolving |
| Catalog No. | RESK-0279 |
| Description | PEI 40000 is a highly charged cationic polymer with a molecular weight of 40,000 that readily binds negatively charged nucleic acid molecules to form complexes and mediates their entry into cells. It is a transient transfection reagent with low cytotoxicity and high transfection efficiency, delivering high gene expression efficiency in HEK293 and CHO cells. Linear PEI has been validated for a broad range of cell lines, including HEK-293, HEK293T, CHO-K1, COS-1, COS-7, NIH/3T3, Sf9, HepG2, and HeLa cells, with transfection efficiencies of 80%-90%. The fully deacylated polymer backbone ensures consistently high performance. This product is the rapid-dissolving type: it dissolves directly in distilled water without pH adjustment, is quick to dissolve, and is convenient to prepare, making it well suited for large-scale transfection. |
| Intended Use | For research use in the transfection of DNA into adherent and suspension cells, including HEK-293, HEK293T, CHO-K1, COS-1, COS-7, NIH/3T3, Sf9, HepG2, and HeLa; suitable for transient and stable transfection. Not suitable for single-stranded RNA transfection. |
| Principle / Technology | Linear PEI is a highly charged cationic polymer that condenses negatively charged nucleic acids into complexes via electrostatic interaction. After cellular uptake by endocytosis, the abundant amine groups of PEI buffer the endosomal environment and trigger the proton sponge effect, promoting endosomal escape and release of the nucleic acid cargo for expression. |
| Sample Type | DNA; adherent and suspension cell lines including HEK-293, HEK293T, CHO-K1, COS-1, COS-7, NIH/3T3, Sf9, HepG2, and HeLa |
| Performance Range / Specifications | Molecular weight: 40,000 Transfection efficiency of 80%-90% in validated cell lines At 70%-80% cell confluency, DNA transfection efficiency exceeds 80% GFP plasmid transfection of adherent HEK-293 cells: 90% efficiency at 72 hours GFP plasmid transfection of suspension CHO-K1 cells: 80% efficiency at 72 hours Fully deacylated structure for consistent high performance Rapid-dissolving: dissolves directly in distilled water |
| Specificity | Suitable for DNA transfection; not suitable for single-stranded RNA transfection |
| Reaction Conditions / Protocol | Dissolve the powder directly in distilled water; no pH adjustment is required. Prepare PEI-DNA complexes in serum-free medium, then add the complexes directly to cells in serum-containing medium. If the medium was changed before transfection, no further medium change is needed; if required, change the medium 6-8 hours after transfection. Transfect cells at 70%-80% confluency for optimal efficiency (above 80%). |
| Components / Formulation | Linear polyethylenimine (PEI), MW 40,000, rapid-dissolving type; fully deacylated polymer backbone |
| Storage Conditions | Store the powder sealed at room temperature. Store the stock solution at 4 °C; freezing of the stock solution is not recommended. |
| Shelf Life | Powder: 2 years when stored sealed at room temperature Stock solution: 3 months at 4 °C |
| Package Specifications | 5 mg 100 mg 1 g 10 g |
| Product Form | Solid (powder), rapid-dissolving |
| Quality Control | Transfection performance verified by GFP plasmid transfection: 90% efficiency in adherent HEK-293 cells and 80% in suspension CHO-K1 cells at 72 hours post-transfection |
| Molecular Weight | 40,000 |
| Source / Origin | Synthetic cationic polymer |
| pH Range / Optimal pH | No pH adjustment required; dissolves directly in distilled water |
| Expiration Date / Stability | Powder is valid for 2 years when stored sealed at room temperature. Stock solution is valid for 3 months at 4 °C; freezing the stock solution is not recommended. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic procedures. |
| Compatibility | Compatible with serum-containing medium at the cell-addition step; complex formation should be performed in serum-free medium. Suitable for adherent and suspension cells and for transient and stable transfection. |
| Recommended Buffer System | Prepare PEI-DNA complexes in serum-free medium |
| Application Notes / Precautions | Not suitable for single-stranded RNA transfection, which will result in transfection failure. Do not freeze the stock solution; store at 4 °C for up to 3 months. Avoid adding excessive plasmid DNA and transfection reagent, which may cause precipitation/flocculation; impurities in low-purity DNA can also cause PEI precipitation. Reduce the DNA amount if precipitation occurs. The reagent is highly efficient and does not require extra dosage. Low cytotoxicity: transfected cells maintain good morphology and express high levels of the target protein. Cost-effective for large-scale transfection. |
| Batch-to-Batch Consistency | Fully deacylated polymer structure ensures consistently high transfection performance across preparations |
For research use only, not for clinical use.
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