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| Product Name | Linear Polyethylenimine (PEI), MW 25,000 |
| Catalog No. | RESK-0278 |
| Description | Linear polyethylenimine (PEI), MW 25,000 is a highly charged cationic polymer that readily binds negatively charged nucleic acid molecules to form complexes and mediates their entry into cells. It is a transient transfection reagent with low cytotoxicity and high transfection efficiency, delivering high gene expression efficiency in HEK293 and CHO cells. Linear PEI has been validated for a broad range of cell lines, including HEK-293, HEK293T, CHO-K1, COS-1, COS-7, NIH/3T3, Sf9, HepG2, and HeLa cells. The reagent is compatible with serum-containing medium and efficiently introduces nucleic acids into cells. During transfection, cytotoxicity is low, cells maintain good morphology, grow normally, and express high levels of the transfected gene product. No complex pretreatment is required; the complex can be added directly to serum-containing medium, reducing handling steps and time. |
| Intended Use | For research use in the transient transfection of nucleic acids (DNA) into mammalian and insect cell lines, including HEK-293, HEK293T, CHO-K1, COS-1, COS-7, NIH/3T3, Sf9, HepG2, and HeLa cells. |
| Principle / Technology | Linear PEI is a highly charged cationic polymer that condenses negatively charged nucleic acids into complexes via electrostatic interaction. After cellular uptake by endocytosis, the abundant amine groups of PEI buffer the endosomal environment and trigger the proton sponge effect, promoting endosomal escape and release of the nucleic acid cargo into the cytoplasm for expression. |
| Sample Type | Nucleic acids (DNA); mammalian and insect cell lines including HEK-293, HEK293T, CHO-K1, COS-1, COS-7, NIH/3T3, Sf9, HepG2, and HeLa |
| Performance Range / Specifications | Appearance: white to yellow solid Molecular weight: 25,000 Melting point: 73-75 °C Solubility: soluble in hot water, cold water at low pH, methanol, and ethanol; insoluble in benzene, diethyl ether, and acetone High gene expression efficiency in HEK293 and CHO cells Low cytotoxicity; compatible with serum-containing medium |
| Reaction Conditions / Protocol | Dissolve the powder in hot water, or in cold water adjusted to a low (acidic) pH to aid dissolution; after dissolution, adjust the pH back to neutral with NaOH to prepare the stock solution. Form PEI-nucleic acid complexes and add them directly to cells in serum-containing medium; no complex pretreatment is required. |
| Components / Formulation | Linear polyethylenimine (PEI), MW 25,000; CAS No. 9002-98-6, 26913-06-4; molecular formula (CH2CH2NH)n |
| Storage Conditions | Powder: store at room temperature or 4 °C. Stock solution: store at -20 °C for long-term storage, or at 4 °C for short-term storage. Do not refreeze the stock solution. |
| Shelf Life | Powder: 2 years at room temperature or 4 °C Stock solution: 1 year at -20 °C; 2 weeks at 4 °C |
| Package Specifications | 1 g 5 x 1 g |
| Product Form | White to yellow solid (powder) |
| Molecular Weight | 25,000 |
| Source / Origin | Synthetic cationic polymer; CAS No. 9002-98-6, 26913-06-4 |
| pH Range / Optimal pH | Dissolve in cold water adjusted to acidic pH, then neutralize to neutral pH with NaOH before use |
| Shipping Conditions | Shipped at room temperature |
| Expiration Date / Stability | Powder is stable for 2 years at room temperature or 4 °C. Stock solution is stable for 1 year at -20 °C or 2 weeks at 4 °C. Do not refreeze the stock solution. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic procedures. |
| Compatibility | Compatible with serum-containing medium; validated in HEK-293, HEK293T, CHO-K1, COS-1, COS-7, NIH/3T3, Sf9, HepG2, and HeLa cells |
| Application Notes / Precautions | Do not refreeze the stock solution. The polymer is insoluble in benzene, diethyl ether, and acetone; use hot water or acidified cold water for dissolution. Low cytotoxicity allows cells to grow normally and express high levels of target protein after transfection. |
For research use only, not for clinical use.
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