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| Product Name | α-Ketoglutaric Acid (α-KG) Ccontent Assay Kit, 50T/48S |
| Catalog No. | SCAK-YJL-0243 |
| Detection Method | UV spectrophotometry |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Alpha-ketoglutarate (α-KG) is a central metabolite at the intersection of the TCA cycle, amino acid metabolism (transamination), and nitrogen balance. It serves as a key substrate for 2-oxoglutarate-dependent dioxygenases, enzymes involved in collagen synthesis, hypoxia sensing (via PHDs), and epigenetic regulation (via TET and JmjC-domain histone demethylases). This kit uses a highly specific enzymatic reaction where α-KG is quantitatively converted to NADH, which is then measured colorimetrically, offering precise quantification in biological fluids and cell/tissue extracts. |
| Product Application | • Monitoring TCA cycle flux and metabolic perturbations in cancer, aging, or nutrient stress. • Research on glutaminolysis and glutamate-glutamine metabolism. • Studies of epigenetics, particularly related to TET protein function and DNA/histone demethylation. • Investigation of osteoblast differentiation and collagen synthesis. |
| Product Advantages | • Enzymatic Specificity: Utilizes α-KG-specific dehydrogenase, virtually eliminating cross-reactivity with other keto acids like pyruvate or oxaloacetate. • High Accuracy & Precision: The coupled enzymatic method provides excellent reproducibility for detecting physiological concentration changes. • Suitable for Diverse Samples: Works reliably with serum, cell culture media, and tissue/cell lysates. • Straightforward Protocol: A fixed endpoint assay format simplifies operation and reduces instrument requirements. |
For research use only, not for clinical use.
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