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| Product Name | Isocitrate Dehydrogenase Mitochondrial (ICDHm) Activity Assay Kit, 50T/24S |
| Catalog No. | SCAK-YJL-0216 |
| Detection Method | Visible spectrophotometry, Spectrophotometer |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Mitochondrial NAD⁺-dependent isocitrate dehydrogenase (ICDHm) catalyzes the oxidative decarboxylation of isocitrate to α-ketoglutarate, generating NADH and CO₂. This reaction is a key regulatory and rate-limiting step in the tricarboxylic acid (TCA) cycle and is crucial for energy production and biosynthetic precursor supply. ICDHm activity is regulated by ADP (activator) and ATP/NADH (inhibitors), linking it directly to cellular energy status. This kit directly measures the formation of NADH at 340 nm. |
| Product Application | • Studies of mitochondrial function, TCA cycle flux, and cellular respiration. • Research on cancer metabolism, neurodegenerative diseases, and aging. • Investigation of redox balance and NAD⁺/NADH ratios. • Assessment of metabolic adaptations in response to nutrients or stress. |
| Product Advantages | • Direct Activity Measurement: Monitors the intrinsic production of NADH by ICDHm, providing a straightforward and specific assay without complex coupling systems. • Mitochondrial Fraction Compatible: Optimized for use with purified mitochondrial preparations or cell/tissue lysates, allowing specific assessment of the mitochondrial enzyme isoform. • Ideal for Regulatory Studies: The assay conditions facilitate the study of allosteric regulation by adding effectors like ADP, ATP, or Ca²⁺. • High Specificity: The use of D-isocitrate as the substrate ensures specificity for the NAD⁺-dependent mitochondrial isoform over the cytosolic NADP⁺-dependent isoform. |
For research use only, not for clinical use.
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