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| Product Name | Insect Cell Transfection Reagent |
| Catalog No. | RESK-0277 |
| Description | This high-performance transfection reagent is developed on an innovative polymer technology and is specifically optimized for insect cell experimental systems. Through a unique cationic polymer complex structure, it efficiently encapsulates and protects DNA molecules, including linear baculovirus DNA and plasmid DNA, significantly improving nucleic acid delivery efficiency while avoiding the cytotoxicity issues that may arise with traditional calcium phosphate or liposomal transfection methods. The reagent offers exceptional broad-spectrum adaptability, achieving stable and highly reproducible transfection in mainstream insect cell lines such as Sf9, Sf21, and High Five, with transfection efficiencies of 85%-95% and no significant impact on cell viability or subsequent protein expression. Its breakthrough formulation supports both serum-containing and serum-free culture systems without tedious medium replacement steps, greatly simplifying the experimental workflow. Whether for rapid construction of recombinant baculoviruses, large-scale production of recombinant proteins, gene function studies, or virus-like particle (VLP) preparation, efficient gene delivery is achieved within 24-48 hours, and exogenous gene expression can persist for more than 72 hours. |
| Intended Use | For research use in the transfection of baculovirus DNA and plasmid DNA into insect cells (e.g., Sf9, Sf21, High Five) for recombinant baculovirus construction, recombinant protein production, gene function studies, and virus-like particle (VLP) preparation. |
| Principle / Technology | A cationic polymer forms complexes with negatively charged DNA molecules, encapsulating and protecting them. The polymer-DNA complexes are efficiently internalized by insect cells, enabling high-efficiency gene delivery with low cytotoxicity. |
| Sample Type | Linear baculovirus DNA and plasmid DNA; insect cell lines including Sf9, Sf21, and High Five |
| Performance Range / Specifications | Transfection efficiency of 85%-95% (above 90% in optimized settings) in insect cell lines Validated in Sf9, Sf21, and High Five cells with stable, reproducible results Compatible with both baculovirus DNA and plasmid DNA Efficient gene delivery within 24-48 hours; exogenous gene expression sustained for over 72 hours Supports serum-containing and serum-free culture systems without medium replacement No significant impact on cell viability or protein expression |
| Specificity | Specifically optimized for insect cell systems |
| Reaction Conditions / Protocol | Add the polymer-DNA complexes directly to insect cell cultures; no medium replacement is required. Efficient gene delivery is achieved within 24-48 hours after transfection. Exogenous gene expression persists for more than 72 hours; protein harvest is typically performed around day 5 for expression studies. |
| Components / Formulation | Cationic polymer-based transfection reagent |
| Storage Conditions | Store at 2-8 °C. Do not freeze. |
| Shelf Life | 2 years when stored at 2-8 °C |
| Package Specifications | 25 uL 100 uL 1 mL |
| Product Form | Liquid |
| Quality Control | Performance verified by GFP fluorescence microscopy, luciferase reporter assays, recombinant protein yield measurement, and Western blot analysis of target protein expression (e.g., GFP-6xHis) in Sf9 and High Five cells |
| Expiration Date / Stability | Valid for 2 years when stored at 2-8 °C. Do not freeze. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic procedures. |
| Compatibility | Compatible with serum-containing and serum-free insect cell culture systems; suitable for baculovirus DNA and plasmid DNA transfection |
| Application Notes / Precautions | Do not freeze. No medium change is needed before or after transfection, simplifying the workflow. Transfected cells show good morphology with no obvious toxicity; high GFP-positive rates and strong target protein expression (verified by luciferase assay and Western blot) are consistently observed. |
For research use only, not for clinical use.
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