- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Insect Cell Transfection Reagent, Baculovirus Expression System |
| Catalog No. | ATR-CTR-0022 |
| Description | Cationic lipid transfection reagent optimized for Sf9, Sf21, and High Five insect cells in baculovirus-based recombinant protein expression. Achieves >90% transfection efficiency with bacmid/plasmid DNA while maintaining low cytotoxicity. Supports transient transfection and baculovirus generation in serum-containing and serum-free insect media. |
| Intended Use | Insect cell transfection for baculovirus generation; recombinant protein expression (Bac-to-Bac, flashBAC); VLP production; multi-gene co-expression in lepidopteran cells. |
| Principle / Technology | Cationic lipid forms positively charged complexes with DNA; optimized for insect cell membrane composition and endocytosis; lipid composition facilitates endosomal escape and nuclear delivery |
| Detection Method | Dilute 1-2 µg DNA in transfection medium; add 3-6 µL reagent; complex 15-30 min; add to cells (60-80% confluent); incubate 4-6 hr; replace medium; harvest 48-96 hr |
| Sample Type | Sf9, Sf21, Tni (High Five), Drosophila S2 cells |
| Reaction Conditions / Protocol | Complex formation: 15-30 min RT; transfection: 4-6 hr or overnight; expression: 48-96 hr |
| Components / Formulation | Cationic lipid blend with co-lipid helper in proprietary buffer; sterile-filtered |
| Storage Conditions | 2-8 °C; do not freeze; warm to RT before use; protect from light |
| Shelf Life | 12 months; 6 months after opening |
| Package Specifications | 0.5 mL, 1 mL, 5 mL vials |
| Product Form | Clear slightly opalescent sterile liquid |
| Key Features | >90% Sf9 transfection efficiency; low cytotoxicity for 96 hr expression; compatible with serum and serum-free media; validated Bac-to-Bac/flashBAC/MultiBac; no sonication needed |
| Purity | Lipid ≥98% TLC; endotoxin <0.1 EU/mL; particle size 150-250 nm after complexation |
| Concentration | As specified (typically 1 mg/mL for lipid reagents) |
| Activity / Unit Definition | Transfection efficiency: ≥70% in HEK293 cells with control plasmid at recommended ratio |
| Molecular Weight | Varies by formulation as specified in product documentation |
| Source / Origin | Synthetic cationic lipids and polymers; recombinant viral components where applicable |
| pH Range / Optimal pH | Lipoplex formation optimal at pH 7.0–7.4 |
| Shipping Conditions | Cold pack 2-8 °C; do not freeze; protect from light |
| Expiration Date / Stability | 12 months at 2-8 °C; 6 months after opening; discard if phase-separated |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with serum-containing and serum-free culture media; check product-specific compatibility |
| Recommended Buffer System | Serum-free medium or PBS for complex formation; complete medium for post-transfection culture |
| Application Notes / Precautions | Optimize reagent-to-DNA ratio per cell type; avoid antibiotic during transfection; monitor cytotoxicity |
| Batch-to-Batch Consistency | Transfection efficiency within ±15% of reference lot on HEK293 standard |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
