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| Product Name | in vitro siRNA/miRNA Transfection Reagent |
| Catalog No. | RESK-0223 |
| Description | This in vitro siRNA/miRNA transfection reagent is a non-liposomal, PEI-based cationic amphiphilic molecule developed for transfecting siRNA into mammalian cells, and is suitable for both siRNA and miRNA transfection. The reagent encapsulates siRNA or miRNA to form cationic complexes that interact with negatively charged proteoglycans on the cell surface, adsorb to the cell surface, and enter cells by endocytosis to form endosomes. Acting as a proton sponge, the reagent absorbs H+ within lysosomes; the continuous influx of protons causes the complex to swell and rupture, releasing the exogenous siRNA or miRNA into the cytoplasm. The product achieves over 90% siRNA delivery efficiency across a wide range of cell lines while avoiding off-target effects. It is suitable for transfection of many cell types, including adherent cells such as HeLa, MCF-7, HepG2, and CHO; hard-to-transfect suspension cell lines such as K562 and THP-1, reaching 80% silencing efficiency; and primary cells such as primary human fibroblasts and primary human hepatocytes, also reaching 80% silencing efficiency. |
| Intended Use | For in vitro transfection of siRNA and miRNA into mammalian cells for gene silencing or regulation studies. Suitable for adherent cell lines (e.g., HeLa, MCF-7, HepG2, CHO), hard-to-transfect suspension cell lines (e.g., K562, THP-1), immune cells, and primary cells (e.g., primary human fibroblasts and hepatocytes). |
| Principle / Technology | Non-liposomal PEI-based cationic amphiphilic transfection reagent. It packages siRNA/miRNA into cationic complexes that bind negatively charged cell-surface proteoglycans, enter cells via endocytosis, and exploit a proton sponge effect to rupture endosomes/lysosomes and release the nucleic acid cargo into the cytoplasm. |
| Sample Type | siRNA and miRNA; mammalian cells including adherent cell lines, suspension cell lines (including immune cells), and primary cells. |
| Performance Range / Specifications | Ultra-high transfection efficiency: delivers siRNA and miRNA into cells with over 90% siRNA delivery efficiency across a wide range of cell lines Approximately 80% silencing efficiency in hard-to-transfect suspension cell lines (e.g., K562, THP-1) and primary cells (e.g., primary human fibroblasts, primary human hepatocytes) Extremely low cytotoxicity: minimizes cell damage and maintains normal physiological function, viability, and intracellular homeostasis Broad cell applicability: suitable for many cell types, including hard-to-transfect cell lines such as immune cells |
| Storage Conditions | Store at 2-8 °C. Do not freeze. |
| Shelf Life | 1 year when stored at 2-8 °C. |
| Package Specifications | 0.1 mL 0.5 mL 1 mL |
| Product Form | Liquid solution. |
| Shipping Conditions | Shipped on wet ice (ice packs). |
| Expiration Date / Stability | Valid for 1 year at 2-8 °C; do not freeze. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic or therapeutic procedures. |
| Compatibility | Compatible with siRNA and miRNA; validated across adherent cell lines (HeLa, MCF-7, HepG2, CHO, etc.), suspension cell lines (K562, THP-1, etc.), immune cells, and primary cells. |
| Application Notes / Precautions | Medium change is permitted 8 h after transfection; however, medium change is generally not recommended because the reagent has very low cytotoxicity and transfection efficiency can be improved without medium change. Do not freeze the product; store at 2-8 °C. When using fluorescently labeled siRNA, successfully transfected cells show whole-cell fluorescence with clear cell morphology, whereas untransfected samples show only fluorescent debris. |
For research use only, not for clinical use.
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