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| Product Name | Hydroxyl-Terminated Magnetic Beads for Nucleic Acid Extraction (300 nm) |
| Catalog No. | MAGBEA-0023 |
| Description | Silica-based magnetic nanobeads with a dense hydroxyl surface coating optimized for high-capacity nucleic acid binding in chaotropic salt-based extraction protocols. The high surface density of hydroxyl groups provides extensive hydrogen bonding sites for DNA and RNA capture, yielding high binding capacity suitable for samples with high nucleic acid content. The narrow particle size distribution ensures consistent magnetic separation behavior across batches. |
| Intended Use | High-capacity magnetic bead-based nucleic acid purification from biological samples in manual and automated workflows. |
| Principle / Technology | Hydrogen bonding of nucleic acids to silica hydroxyl surface under chaotropic salt conditions |
| Detection Method | Spectrophotometric quantification at 260 nm; gel electrophoresis |
| Sample Type | Whole blood, tissue homogenates, cultured cells, bacteria, plant tissue, body fluids |
| Performance Range / Specifications | DNA binding capacity: ≥5 µg per mg beads; particle diameter: 300 ± 30 nm; surface area: ≥50 m²/g |
| Sensitivity / LOD | DNA recovery: ≥90% for 200 bp–50 kb fragments from whole blood extraction |
| Specificity | Binds single-stranded and double-stranded DNA and RNA non-selectively under chaotropic conditions |
| Reaction Conditions / Protocol | Mix sample lysate with binding buffer and bead suspension; incubate 5 min with gentle mixing; magnetically separate 2 min; wash with 70% ethanol 2×; air dry 5 min; elute with Tris buffer or water |
| Components / Formulation | Hydroxyl magnetic bead suspension (50 mg/mL in storage solution), binding buffer concentrate, wash buffer, elution buffer |
| Storage Conditions | Beads: room temperature; buffers: room temperature |
| Shelf Life | 24 months at room temperature |
| Package Specifications | 10 mL bead suspension (500 mg beads), sufficient for 500–1000 extractions |
| Product Form | Magnetic bead suspension, dark brown |
| Quality Control | DNA binding capacity verified with human genomic DNA; particle size distribution confirmed by dynamic light scattering; lot-to-lot CV of binding capacity ≤10% |
| Key Features | High hydroxyl surface density provides nearly twice the nucleic acid binding capacity of standard silica beads |
| Purity | Endotoxin ≤0.1 EU/mg; heavy metal content ≤10 ppm |
| Concentration | As specified (mg/mL suspension) |
| Activity / Unit Definition | Binding capacity in µg target per mg beads as specified |
| Molecular Weight | Not applicable — inorganic/organic composite particles |
| Source / Origin | Synthetic polymer-magnetite composite; recombinant protein ligands where applicable |
| pH Range / Optimal pH | pH 6.0–9.0 working range; optimal pH varies by surface chemistry |
| Shipping Conditions | Ambient temperature; protect from freezing for aqueous suspensions |
| Expiration Date / Stability | 18–24 months at recommended storage temperature |
| Regulatory / Compliance | Research use; ISO 9001 manufacturing; custom GMP options available |
| Compatibility | Compatible with manual magnetic racks and automated KingFisher and similar platforms |
| Recommended Buffer System | PBS pH 7.4 with 0.02% sodium azide or 20% ethanol for long-term storage |
| Application Notes / Precautions | Vortex thoroughly before each use; avoid drying beads; use appropriate magnetic separation times |
| Batch-to-Batch Consistency | Particle size CV ≤10%; binding capacity within ±15% of reference lot |
For research use only, not for clinical use.
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