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| Product Name | Hybridisation and Wash Kit with Universal Blocking Oligo for mainstream sequencing Platforms |
| Catalog No. | REKI-0053 |
| Description | A hybridisation and wash reagent kit developed for targeted capture workflows on mainstream sequencing platforms including NovaSeq 6000. Paired with novel 100-mer double-stranded DNA probes, the optimised single-tube hybridisation buffer system yields stable, high-quality target capture with strong sensitivity and specificity for molecular diagnostics research. |
| Intended Use | Target enrichment supporting tumour companion diagnostics, therapy assessment, hereditary screening, molecular breeding and molecular diagnostics development. |
| Principle / Technology | Biotinylated probes hybridise to targets, streptavidin capture isolates hybrids, and wash buffers strip off-target DNA before sequencing. |
| Performance Range / Specifications | Single-tube operation; bead-based concentration may replace a vacuum concentrator |
| Specificity | Accepts both DNA and RNA capture probes of 100-mer design; blocking oligo matched to mainstream sequencing adapters |
| Reaction Conditions / Protocol | Combine library, probes and blocking oligo, hybridise, capture, wash and amplify the enriched pool |
| Components / Formulation | Hybridisation buffer, wash buffers and universal blocking oligo; capture probes ordered separately |
| Storage Conditions | BOX I and BOX III: -25 to -15°C; BOX II: room temperature |
| Shelf Life | 1 year |
| Package Specifications | 4 T / 24 T / 96 T |
| Product Form | Three-box kit with temperature-separated modules |
| Key Features | Single-tube chemistry; low equipment dependency; dual DNA/RNA probe compatibility |
| Compatibility | Compatible with mainstream sequencing platforms including NovaSeq 6000 and matched probe panels |
For research use only, not for clinical use.
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