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| Product Name | Hybridisation and Wash Kit with Universal Blocking Oligo for mainstream sequencing Platforms |
| Catalog No. | REKI-0052 |
| Description | A hybridisation and wash reagent kit developed for targeted capture workflows on mainstream sequencing platforms. Paired with novel 100-mer double-stranded DNA probes, the optimised single-tube hybridisation buffer system delivers stable, high-quality capture of target DNA for high-throughput sequencing, with excellent sensitivity and specificity in emerging molecular diagnostics research. |
| Intended Use | Target enrichment for tumour companion diagnostics, therapy assessment, hereditary disease screening, molecular breeding and molecular diagnostics research. |
| Principle / Technology | Biotinylated probes hybridise to target regions; streptavidin-based capture isolates the hybrids, and wash chemistry removes off-target material before sequencing. |
| Sample Type | Libraries prepared from genomic DNA |
| Performance Range / Specifications | Single-tube operation; vacuum concentrator can be replaced by bead-based concentration |
| Specificity | Compatible with both DNA and RNA capture probes of 100-mer design |
| Reaction Conditions / Protocol | Pool library with probes and blocking oligo, hybridise, capture with streptavidin beads, wash and amplify |
| Components / Formulation | Hybridisation buffer, wash buffers and universal blocking oligo; capture probes are ordered separately |
| Storage Conditions | BOX I: -25 to -15°C; BOX II: room temperature; BOX III: -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 4 T / 24 T / 96 T |
| Product Form | Three-box kit with temperature-separated modules |
| Key Features | Single-tube hybridisation chemistry; low equipment dependency; supports DNA and RNA probe panels |
| Compatibility | Compatible with mainstream sequencing platforms and matched targeted capture probe panels |
For research use only, not for clinical use.
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