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| Product Name | Hybrid Reagent and Column Total RNA Isolation Kit |
| Catalog No. | DREK-0111 |
| Description | A hybrid total RNA isolation workflow that combines strong phenol-guanidinium sample lysis with selective silica-membrane purification. It offers broad sample compatibility and delivers purified RNA without relying solely on precipitation-based cleanup. |
| Intended Use | Total RNA isolation for RT-PCR, qRT-PCR, Northern blotting, dot blotting, in vitro translation and high-throughput sequencing. |
| Principle / Technology | Phenol-guanidinium lysis and phase separation followed by selective silica-membrane RNA binding, washing and elution. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Cultured cells, animal tissues, plant tissues and fresh whole blood. |
| Performance Range / Specifications | Approximately 20-minute workflow performed with centrifugation at a consistent temperature; 100 reactions. |
| Reaction Conditions / Protocol | Lyse the sample using the supplied single-phase reagent, recover the RNA-containing aqueous phase, load onto the silica column, wash and elute. |
| Components / Formulation | Phenol-guanidinium lysis reagent and silica spin-column purification components. |
| Package Specifications | 100 reactions. |
| Product Form | Hybrid reagent and spin-column total RNA isolation kit. |
| Key Features | High RNA yield and purity; strong lysis; broad compatibility; simplified column cleanup; approximately 20-minute workflow. |
| Compatibility | RT-PCR, qRT-PCR, blotting, in vitro translation and sequencing workflows. |
| Application Notes / Precautions | Handle the lysis reagent using suitable protective equipment and avoid disturbing the interphase during aqueous-phase recovery. |
For research use only, not for clinical use.
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