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| Product Name | Human/Mouse/Rat rRNA and ITS/ETS Depletion Kit (RNase H Method) |
| Catalog No. | REKI-0055 |
| Description | An RNase H digestion kit that removes ribosomal RNA and 45S ITS/ETS regions from human, mouse and rat total RNA to preserve messenger RNA and other non-coding RNA. Dedicated probes against the human, mouse and rat 45S ITS/ETS regions address the elevated ITS/ETS fraction typical of degraded FFPE samples, lifting the useful data proportion markedly after removal. |
| Intended Use | rRNA removal in RNA sequencing library workflows for regular and variable-quality FFPE samples. |
| Principle / Technology | Probe-guided RNase H digests rRNA and ITS/ETS spacer transcripts while sparing mRNA and non-coding RNA. |
| Detection Method | Cytoplasmic rRNA and 45S ITS/ETS regions of human, mouse and rat |
| Sample Type | Total RNA and FFPE-derived RNA |
| Performance Range / Specifications | Low rRNA residual rate after treatment; effective on intact and partially degraded RNA |
| Specificity | ITS/ETS probe panel matched to human, mouse and rat 45S regions |
| Storage Conditions | Store at -25 to -15°C |
| Package Specifications | 12 T / 24 T / 96 T |
| Product Form | Kit with RNase H and probe modules |
| Key Features | ITS/ETS coverage for FFPE samples; broad human, mouse and rat compatibility; works with downstream library kits from any supplier |
| Compatibility | Adaptable to different brands of RNA library prep kits downstream |
For research use only, not for clinical use.
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