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| Product Name | High-Sensitivity Probe qPCR Master Mix |
| Catalog No. | NATR-HMM-0198 |
| Description | A high-sensitivity probe qPCR master mix using an antibody-mediated hot-start polymerase with enhanced template affinity and tolerance to blood-related inhibitors. |
| Intended Use | Viral nucleic acid detection; high-sensitivity probe qPCR; gene-expression analysis. |
| Principle / Technology | An optimized hot-start DNA polymerase and probe-qPCR buffer support efficient sequence-specific amplification from low-template or inhibitor-containing DNA samples. |
| Detection Method | Fluorescent probe-based real-time PCR. |
| Sample Type | DNA templates, including low-input samples and samples containing blood-related impurities. |
| Performance Range / Specifications | 500 reactions at 20 µL per reaction; supports fast programs and a broad range of template types, GC content and primer melting temperatures. |
| Sensitivity / LOD | Optimized for low-template and high-sensitivity detection. |
| Specificity | Probe-based detection and antibody-mediated hot-start control support sequence-specific amplification. |
| Reaction Conditions / Protocol | Combine the 2× master mix with primers, probe and DNA template, then run a compatible standard or fast probe qPCR program. |
| Components / Formulation | 2× probe qPCR master mix containing antibody-mediated hot-start DNA polymerase and optimized reaction buffer. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 500 reactions (20 µL per reaction). |
| Product Form | 2× high-sensitivity probe qPCR master mix. |
| Key Features | High template affinity; low-template sensitivity; blood-related inhibitor tolerance; fast cycling; broad assay compatibility. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with common fluorescent probe-based real-time PCR instruments and assays across broad target GC and primer-melting-temperature conditions. |
For research use only, not for clinical use.
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