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| Product Name | High-Sensitivity Multiplex Probe qPCR Premix |
| Catalog No. | NATR-HMM-0194 |
| Description | A high-sensitivity multiplex probe qPCR premix for DNA templates, designed for robust amplification across broad GC content and impurity-containing samples. |
| Intended Use | Multiplex quantitative PCR; pathogen detection; animal-disease testing; biopharmaceutical quality control. |
| Principle / Technology | A high-affinity hot-start DNA polymerase, optimized multiplex buffer and dUTP/UDG carryover-control system support simultaneous probe-based amplification of multiple DNA targets. |
| Detection Method | Multiplex fluorescent probe-based real-time PCR. |
| Sample Type | DNA templates, including samples containing blood-related inhibitors, residual ethanol or guanidinium salts. |
| Performance Range / Specifications | 200, 500 or 2,500 reactions; compatible with templates spanning approximately 20–75% GC content; supports fast programs completed in 30 minutes or less. |
| Sensitivity / LOD | Optimized for high-sensitivity multiplex detection. |
| Specificity | Strong 3′-end mismatch discrimination and hot-start control support specific multiplex amplification. |
| Reaction Conditions / Protocol | Combine the 2× premix with primers, probes and DNA template, then run a compatible multiplex probe qPCR program. |
| Components / Formulation | 2× multiplex probe qPCR premix containing high-affinity hot-start DNA polymerase and a dUTP/UDG carryover-control system. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 500 reactions; 2,500 reactions. |
| Product Form | 2× multiplex probe qPCR premix. |
| Quality Control | Multiplex sensitivity, specificity, inhibitor tolerance and accelerated primer/probe premix stability are evaluated by real-time PCR. |
| Key Features | High sensitivity; strong mismatch discrimination; inhibitor tolerance; broad GC compatibility; fast cycling; carryover control. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Primer/probe premixes prepared with the system remained stable for 28 days at 37°C and through 50 freeze–thaw cycles under the evaluated accelerated conditions. |
| Compatibility | Suitable for multiplex DNA-target assays and common probe-based real-time PCR platforms. |
For research use only, not for clinical use.
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