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| Product Name | High-Efficiency Polymer Transfection Reagent |
| Catalog No. | RESK-0217 |
| Description | This reagent is an upgraded nucleic acid transfection reagent developed on a novel polymeric macromolecular material rather than a conventional liposome. Its unique molecular design markedly reduces cytotoxicity, and an intelligent anti-enzymatic-degradation mechanism efficiently protects nucleic acids from intracellular enzymatic damage during delivery, maximizing transfection efficiency. Unlike liposomal transfection reagents, it exhibits very low cytotoxicity and can gently transfect even fragile cells. The reagent is broadly compatible with multiple nucleic acid types, including DNA, siRNA, miRNA, mRNA, and ASO, and has been validated for high-efficiency transfection in 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, HCT116, and primary cells, providing a reliable tool for gene research. |
| Intended Use | For research use in the intracellular delivery of DNA, siRNA, miRNA, mRNA, and antisense oligonucleotides (ASO) into mammalian cells, including common cell lines, sensitive cells, and primary cells. |
| Principle / Technology | A novel cationic polymeric macromolecule replaces traditional liposomes to condense and deliver nucleic acids, reducing cytotoxicity and improving survival of sensitive and primary cells. A specific anti-enzymatic-degradation mechanism (lysosomal escape combined with protective shielding of the nucleic acid cargo) preserves intracellular nucleic acid integrity and increases transfection efficiency. |
| Sample Type | Cultured mammalian cells, including common cell lines, sensitive cells, and primary cells. |
| Performance Range / Specifications | Transfection efficiency >90% (measured by flow cytometry). Covers delivery of all nucleic acid types: DNA, siRNA, miRNA, mRNA, and ASO; efficiently transfects large fragments up to 15 kb. High-efficiency transfection validated in 30+ cell lines, including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116, with high transfection efficiency also achieved in primary cells. |
| Components / Formulation | Ready-to-use liquid formulation based on a novel cationic polymeric macromolecular material. |
| Storage Conditions | Store at 2–8 °C. |
| Shelf Life | 1 year when stored at 2–8 °C. |
| Package Specifications | 25 uL 100 uL 0.75 mL 1.5 mL |
| Product Form | Liquid |
| Compatibility | Compatible with DNA, siRNA, miRNA, mRNA, and ASO. Validated in 30+ cell lines including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116, as well as primary cells. |
| Application Notes / Precautions | Very low cytotoxicity; suitable for gentle transfection of fragile and sensitive cells. Higher intracellular nucleic acid integrity owing to the lysosomal escape and cargo-protection mechanism. In side-by-side testing across multiple cell lines, the reagent achieved superior DNA transfection efficiency, mRNA transfection efficiency, and siRNA-mediated gene knockdown. Suitable for transfection of large nucleic acid fragments up to 15 kb. |
For research use only, not for clinical use.
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